Salicylate Functions as an Efflux Pump Inducer and Promotes the Emergence of Fluoroquinolone-Resistant Campylobacter jejuni Mutants

dc.contributor.author Shen, Zhangqi
dc.contributor.author Pu, Xiao-Ying
dc.contributor.author Zhang, Qijing
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-18T23:26:46.000
dc.date.accessioned 2020-07-07T05:14:42Z
dc.date.available 2020-07-07T05:14:42Z
dc.date.copyright Sat Jan 01 00:00:00 UTC 2011
dc.date.issued 2011-10-01
dc.description.abstract <p>Salicylate, a nonsteroidal anti-inflammatory compound, has been shown to increase the resistance of Campylobacter to antimicrobials. However, the molecular mechanism underlying salicylate-induced resistance has not yet been established. In this study, we determined how salicylate increases antibiotic resistance and evaluated its impact on the development of fluoroquinolone-resistant Campylobacter mutants. Transcriptional fusion assays, real-time quantitative reverse transcription-PCR (RT-PCR), and immunoblotting assays consistently demonstrated the induction of the CmeABC multidrug efflux pump by salicylate. Electrophoretic mobility shift assays further showed that salicylate inhibits the binding of CmeR (a transcriptional repressor of the TetR family) to the promoter DNA of <em>cmeABC</em>, suggesting that salicylate inhibits the function of CmeR. The presence of salicylate in the culture medium not only decreased the susceptibility of Campylobacter to ciprofloxacin but also resulted in an approximately 70-fold increase in the observed frequency of emergence of fluoroquinolone-resistant mutants under selection with ciprofloxacin. Together, these results indicate that in Campylobacter, salicylate inhibits the binding of CmeR to the promoter DNA and induces expression of <em>cmeABC</em>, resulting in decreased susceptibility to antibiotics and in increased emergence of fluoroquinolone-resistant mutants under selection pressure.</p>
dc.description.comments <p>This article is published as Shen, Zhangqi, Xiao-Ying Pu, and Qijing Zhang. "Salicylate functions as an efflux pump inducer and promotes the emergence of fluoroquinolone-resistant Campylobacter jejuni mutants." Applied and environmental microbiology 77, no. 20 (2011): 7128-7133. doi: <a href="http://dx.doi.org/10.1128/AEM.00763-11%20" target="_blank" title=" Salicylate Functions as an Efflux Pump Inducer and Promotes the Emergence of Fluoroquinolone-Resistant Campylobacter jejuni Mutants">10.1128/AEM.00763-11</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/164/
dc.identifier.articleid 1164
dc.identifier.contextkey 10836590
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/164
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92270
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/164/2011_Zhang_SalicylateFunctions.pdf|||Fri Jan 14 20:59:42 UTC 2022
dc.source.uri 10.1128/AEM.00763-11
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Molecular Genetics
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Salicylate Functions as an Efflux Pump Inducer and Promotes the Emergence of Fluoroquinolone-Resistant Campylobacter jejuni Mutants
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 1c6a5dfc-c604-457f-85be-122910db782e
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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