Research Notes : United States : A method of isolating poly(A)-containing RNA from soybean suspension culture cells for cDNA synthesis and cloning

Date
1984-04-01
Authors
Brown, J.
Treick, R.
Wilson, K.
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Research Projects
Organizational Units
Series
Department
Iowa State University Digital Repository
Abstract

Our laboratories are interested in using recombinant DNA techniques to study the genetics of amino acid metabolism in soybeans. An especially useful technique used to study gene regulation is the in vitro synthesis of DNA com-plementary to mRNA; this cDNA can be used directly as a hybridization probe, or cloned and amplified in a suitable host. The protocol described here was developed for isolating total cellular poly{A)-containing RNA from soybean suspension culture cells of sufficient purity for use as a template for cDNA synthesis.

Comments
Description
Keywords
Citation
DOI
Source
Collections