This investigation was intended to provide actual data on the seroprevalence of Yersinia antibodies in German slaughtering pigs. The recomB lot western blot assay based on five recombinantly produced Yersinia Outer Proteins (Yap) is well evaluated for human Yersiniosis and proved to be also valuable for the detection of porcine anti-Yap antibodies. 970 out of 1014 meat juice samples collected in 145 Bavarian pig farms contained anti-Yap antibodies. As other animal pathogens like Salmonella express the proteins of secretion apparatus III as well, we propose that only blots with three and more detectable signals should be considered as positive. This means that at least 45.2% of the pigs and 82% of the pig farms respectively had a previous history of Yersinia infection. Yersinia infection in pigs is still a severe hygienical problem. Furtheron, pigs may not only be asymptomatic carriers but may also suffer from disease leading to economical losses.