Mapping of QTL and Identification of Candidate Genes Conferring Spontaneous Haploid Genome Doubling in Maize (Zea mays L.)

Date
2019-11-21
Authors
Ren, Jiaojiao
Boerman, Nicholas
Lubberstedt, Thomas
Liu, Ruixiang
Wu, Penghao
Trampe, Benjamin
Vanous, Kimberly
Frei, Ursula
Chen, Shaojiang
Lubberstedt, Thomas
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Agronomy
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Agronomy
Abstract

In vivo doubled haploid (DH) technology is widely used in commercial maize (Zea mays L.) breeding. Haploid genome doubling is a critical step in DH breeding. In this study, inbred lines GF1 (0.65), GF3(0.29), and GF5 (0) with high, moderate, and poor spontaneous haploid genome doubling (SHGD), respectively, were selected to develop mapping populations for SHGD. Three QTL, qshgd1, qshgd2, and qshgd3, related to SHGD were identified by selective genotyping. With the exception of qshgd3, the source of haploid genome doubling alleles were derived from GF1. Furthermore, RNA-Seq was conducted to identify putative candidate genes between GF1 and GF5 within the qshgd1 region. A differentially expressed formin-like protein 5 transcript was identified within the qshgd1 region.

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This is a manuscript of an article published as Ren, Jiaojiao, Nicholas Boerman, Ruixiang Liu, Penghao Wu, Benjamin Trampe, Kimberly Vanous, Ursula K. Frei, Shaojiang Chen, and Thomas Lübberstedt. "Mapping of QTL and Identification of Candidate Genes Conferring Spontaneous Haploid Genome Doubling in Maize (Zea mays L.)." Plant Science (2019). doi: 10.1016/j.plantsci.2019.110337. Posted with permission.

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