Testing the Efficacy of the Ultra-Filtration Centrifuge for the Stable Isotope Analysis of Bone Collagen
Date
2024-05
Authors
Smadi, Seema
Major Professor
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Somerville, Andrew
Committee Member
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Abstract
Stable isotope analysis (SIA) has become an essential tool in a range of studies, including in ecological and archaeological research. This project assesses the necessity of ultrafiltration units in purifying bone collagen for SIA. While the method results in the reduction of possible contaminants, it adds additional time to sample preparation and can result in greater sample loss. This study analyzes seven ancient bison bones from central Iowa, partitioning each bone into six sub-samples. Half of these sub-samples underwent standard treatment, while the other half received ultrafiltration. The 42 samples were analyzed for stable carbon and nitrogen isotope ratios at the Stable Isotope Laboratory at ISU. The data accuracy is evaluated based on comparisons with results previously obtained from the University of California, Irvine, and by assessing the standard deviations of the results from each bone. The significance of this research is its potential to improve data collection efficiency and reduce costs associated with outsourcing sample processing for radiocarbon dating. Additionally, it contributes to the understanding of whether ultrafiltration is essential for stable isotope analysis. The compilation of acquired knowledge on the ultrafilters gained from this study has been compiled into a manual for future use of the equipment.
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