Culturing techniques are complicated and time consuming by many veterinarians. Control of mastitis is often attempted with out detection of subclinical cases which serve as reservoirs of infection for the entire herd. Effective control of bovine mastitis must necessarily be based upon the detection of every infected quarter in a herd. Identification of the etiologic agents avoids hit or miss methods, gives indications for specific therapy, aids in prognosis and furnishes information for a definite plan of control.