Targeted Microfluidic Manufacturing to Mimic Biological Microenvironments: Cell-Encapsulated Hollow Fibers

Date
2021-05-28
Authors
McNamara, Marilyn
Aykar, Saurabh
Montazami, Reza
Hashemi, Nicole
Hashemi, Nicole
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Ames Laboratory
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Biomedical Sciences
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Mechanical Engineering
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Ames LaboratoryBiomedical SciencesMechanical EngineeringBioeconomy Institute (BEI)
Abstract

At present, the blood–brain barrier (BBB) poses a challenge for treating a wide range of central nervous system disorders; reliable BBB models are still needed to understand and manipulate the transfer of molecules into the brain, thereby improving the efficiency of treatments. In this study, hollow, cell-laden microfibers are fabricated and investigated as a starting point for generating BBB models. The genetic effects of the manufacturing process are analyzed to understand the implications of encapsulating cells in this manner. These fibers are created using different manufacturing parameters to understand the effects on wall thickness and overall diameter. Then, dopaminergic rat cells are encapsulated into hollow fibers, which maintained at least 60% live cells throughout the three-day observation period. Lastly, genetic changes tyrosine hydroxylase (TH) and tubulin beta 3 class III (TUBB-3) are investigated to elucidate the effects on cell health and behavior; while the TH levels in encapsulated cells were similar to control cells, showing similar levels of TH synthesis, TUBB-3 was downregulated, indicating lower amounts of cellular neurogenesis.

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This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in ACS Macro Letters, copyright © American Chemical Society after peer review. To access the final edited and published work see DOI: 10.1021/acsmacrolett.1c00159. Posted with permission.

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