Validation of a first-generation long-oligonucleotide microarray for transcriptional profiling in the pig

Date
2005-11-01
Authors
Zhao, Shu-Hong
Recknor, Justin
Lunney, Joan
Nettleton, Dan
Nettleton, Dan
Kuhar, Daniel
Orley, Sarah
Tuggle, Christopher
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Animal Science
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Statistics
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Animal ScienceStatisticsElectrical and Computer Engineering
Abstract

A first-generation porcine oligonucleotide set, representing 13,297 cDNAs and ESTs, has been designed by Qiagen–Operon for transcriptional profiling. To validate this set, microarrays containing each 70-mer oligonucleotide, referred to as the Qiagen–NRSP8 array, were hybridized with targets from porcine adult liver, lung, muscle, or small intestine. Transcriptome analyses showed that 11,328 of the oligonucleotides demonstrated expression in at least one tissue. Statistical analyses revealed that 1810 genes showed differential expression among tissues (Bonferroni adjusted p < 0.05). Biological pathways identified by DAVID/EASE analysis using a list of 423 tissue-selective genes matched archetypal pathways in the corresponding human or mouse tissue. Real-time quantitative PCR confirmed expression patterns for 9 of 11 genes tested. Our results demonstrate that this first-generation porcine oligonucleotide array is informative and the specificity is high. This is essential validation for investigators using the Qiagen–NRSP8 array for porcine functional genomics and for using the pig in modeling important physiological problems.

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This article is published as Zhao, Shu-Hong, Justin Recknor, Joan K. Lunney, Dan Nettleton, Daniel Kuhar, Sarah Orley, and Christopher K. Tuggle. "Validation of a first-generation long-oligonucleotide microarray for transcriptional profiling in the pig." Genomics 86, no. 5 (2005): 618-625. doi: 10.1016/j.ygeno.2005.08.001.

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