Gradient Separation and Cytochemical Characterisation of Neutrophils from Kidney of Fathead Minnow (Pimephales Promelas Rafinesque, 1820)

dc.contributor.author Andreasen, Claire
dc.contributor.author Palić, Dušan
dc.contributor.author Andreasen, Claire
dc.contributor.author Frank, Dagmar
dc.contributor.author Roth, James
dc.contributor.author Menzel, Bruce
dc.contributor.author Roth, James
dc.contributor.department Veterinary Pathology
dc.date 2018-02-16T19:38:13.000
dc.date.accessioned 2020-07-07T05:16:04Z
dc.date.available 2020-07-07T05:16:04Z
dc.date.issued 2005-03-01
dc.description.abstract <p>Neutrophils are an important component of host defence against many bacterial, viral and fungal infections, and the evaluation of neutrophil function is valuable for assessment of health status [1]. Some literature is non-specific or contradictory about the classification of leucocyte morphology in fish, since morphology alone can vary by species [2e4]. Most studies document that fish neutrophils have very similar morphological and histochemical staining properties to mammalian neutrophils, and can be distinguished by the presence of myeloperoxidase in cytoplasmic granules [5]. Neutrophils are found in fish kidney, spleen and blood, and are commonly increased in inflammatory lesions [6]. There is evidence for phagocytic, chemotactic and bactericidal functions in fish neutrophils and an intense respiratory burst [7e9]. Techniques for obtaining relatively pure neutrophil populations for functional studies have not been previously reported for the fathead minnow (Pimephales promelas Rafinesque, 1820), and the characterisation of leucocyte morphology and cytochemical staining has not been adequately defined.</p>
dc.description.comments <p>This article is from <em>Fish & Shellfish Immunology</em> 18 (2005): 263, doi:<a href="http://dx.doi.org/10.1016/j.fsi.2004.07.003" target="_blank">10.1016/j.fsi.2004.07.003</a>.</p>
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dc.identifier archive/lib.dr.iastate.edu/vpath_pubs/68/
dc.identifier.articleid 1070
dc.identifier.contextkey 7356680
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vpath_pubs/68
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92495
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vpath_pubs/68/2005_Andreasen_GradientSeparation.pdf|||Sat Jan 15 01:28:38 UTC 2022
dc.source.uri 10.1016/j.fsi.2004.07.003
dc.subject.disciplines Natural Resources and Conservation
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords neutrophils
dc.subject.keywords fish
dc.subject.keywords gradient separation
dc.subject.keywords cytochemistry
dc.subject.keywords fathead minnow
dc.subject.keywords Veterinary Microbiology & Preventive Medicine
dc.title Gradient Separation and Cytochemical Characterisation of Neutrophils from Kidney of Fathead Minnow (Pimephales Promelas Rafinesque, 1820)
dc.type article
dc.type.genre article
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relation.isAuthorOfPublication 909dd0b2-ec0a-41e2-b4e0-9e5ff76b7622
relation.isOrgUnitOfPublication cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f
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