Bioallethrin activates specific olfactory sensory neurons and elicits spatial repellency in Aedes aegypti

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Date
2021-10-18
Authors
Valbon, Wilson
Andreazza, Felipe
Oliveira, Eugenio E.
Liu, Feng
Feng, Bo
Hall, Maura
Klimavicz, James
Dong, Ke
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© 2021 Society of Chemical Industry
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Entomology
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Entomology
Abstract
BACKGROUND: Use of pyrethroid insecticides is a pivotal strategy for mosquito control globally. Commonly known for its insecticidal activity by acting on voltage-gated sodium channels, volatile pyrethroids, such as bioallethrin and transfluthrin, are used in mosquito coils, emanators, and other vaporizers to repel mosquitoes and other biting arthropods. However, whether specific olfactory receptor neurons are activated by pyrethroids to trigger spatial repellency remains unknown. RESULTS: We took behavioral and electrophysiological approaches to elucidate the mechanism of bioallethrin repellency in Aedes aegypti, a major vector of dengue, yellow fever, Zika and chikungunya viruses. We found that bioallethrin elicits spatial (i.e., non-contact) repellency and activates a specific type of olfactory receptor neurons in mosquito antennae. Furthermore, bioallethrin repellency is significantly reduced in a mosquito mutant of Orco, an obligate olfactory co-receptor that is essential for the function of odorant receptors (Ors). These results indicate that activation of specific Or(s) by bioallethrin contributes to bioallethrin repellency. In addition, bioallethrin repellency was reduced in a pyrethroid-resistant strain which carries two mutations in the sodium channel gene that are responsible for knockdown resistance (kdr) to pyrethroids, indicating a role of activation of sodium channels in bioallethrin repellency. CONCLUSION: Results from this study show that bioallethrin repellency is likely the result of co-activation of Or(s) and sodium channels. These findings not only contribute to the understanding of the modes of action of volatile pyrethroids in spatial repellency, but also provide a framework for developing new repellents based on the dual-target mechanism revealed.
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This is the peer reviewed version of the following article: Valbon, Wilson, Felipe Andreazza, Eugenio E. Oliveira, Feng Liu, Bo Feng, Maura Hall, James Klimavicz, Joel R. Coats, and Ke Dong. "Bioallethrin activates specific olfactory sensory neurons and elicits spatial repellency in Aedes aegypti." Pest management science (2021), which has been published in final form at DOI:10.1002/ps.6682. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
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Pyrethroids, spatial repellency, olfaction, sodium channels
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