Hepatic transcriptome analyses of juvenile white bass (Morone chrysops) when fed diets where fish meal is partially or totally replaced by alternative protein sources

dc.contributor.author Fuller, S. Adam
dc.contributor.author Abernathy, Jason W.
dc.contributor.author Sankappa, Nithin Muliya
dc.contributor.author Beck, Benjamin H.
dc.contributor.author Rawles, Steven D.
dc.contributor.author Green, Bartholomew W.
dc.contributor.author Rosentrater, Kurt
dc.contributor.author McEntire, Matthew E.
dc.contributor.author Huskey, George
dc.contributor.author Webster, Carl D.
dc.contributor.department Department of Agricultural and Biosystems Engineering (ENG)
dc.date.accessioned 2024-01-16T17:24:21Z
dc.date.available 2024-01-16T17:24:21Z
dc.date.issued 2024-01-15
dc.description.abstract White bass (Morone chrysops) are a popular sportfish throughout the southern United States, and one parent of the commercially-successful hybrid striped bass (M. chrysops ♂ x M. saxatilis ♀). Currently, white bass are cultured using diets formulated for other carnivorous fish, such as largemouth bass (Micropterus salmoides) or hybrid striped bass and contain a significant percentage of marine fish meal. Since there are no studies regarding the utilization of alternative proteins in this species, we evaluated the global gene expression of white bass fed diets in which fish meal was partially or totally replaced by various combinations of soybean meal, poultry by-product meal, canola meal, soy protein concentrate, wheat gluten, or a commercial protein blend (Pro-Cision™). Six isonitrogenous (40% protein), isolipidic (11%), and isocaloric (17.1 kJ/g) diets were formulated to meet the known nutrient and energy requirements of largemouth bass and hybrid striped bass using nutrient availability data for most of the dietary ingredients. One of the test diets consisted exclusively of plant protein sources. Juvenile white bass (40.2 g initial weight) were stocked into a flow-through aquaculture system (three tanks/diet; 10 fish/tank) and fed the test diets twice daily to satiation for 60 days. RNA sequencing and bioinformatic analyses revealed significant differentially expressed genes between all test diets when compared to fish meal control. A total of 1,260 differentially expressed genes were identified, with major ontology relating to cell cycle and metabolic processes as well as immune gene functions. This data will be useful as a resource for future refinements to moronid diet formulation, as marine fish meal becomes limiting and plant ingredients are increasingly added as a reliable protein source.
dc.description.comments This article is published as Fuller, S. Adam, Jason W. Abernathy, Nithin Muliya Sankappa, Benjamin H. Beck, Steven D. Rawles, Bartholomew W. Green, Kurt A. ROSENTRATER, Matthew E. McEntire, George Huskey, and Carl D. Webster. "Hepatic Transcriptome Analyses of Juvenile White Bass (Morone chrysops) when Fed Diets where Fish Meal is Partially or Totally Replaced by Alternative Protein Sources." Frontiers in Physiology 14: 1308690. doi: https://doi.org/10.3389/fphys.2023.1308690. Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/Dw88J3kw
dc.language.iso en
dc.publisher Frontiers in Physiology
dc.source.uri https://doi.org/10.3389/fphys.2023.1308690 *
dc.subject.disciplines DegreeDisciplines::Life Sciences::Animal Sciences::Aquaculture and Fisheries
dc.subject.keywords alternative diets
dc.subject.keywords carnivorous fish
dc.subject.keywords fish meal
dc.subject.keywords plant meal
dc.subject.keywords transcriptomics
dc.subject.keywords RNAseq
dc.subject.keywords white bass
dc.subject.keywords temperate basses
dc.title Hepatic transcriptome analyses of juvenile white bass (Morone chrysops) when fed diets where fish meal is partially or totally replaced by alternative protein sources
dc.type article
dspace.entity.type Publication
relation.isAuthorOfPublication ae6468d9-2286-48ad-9293-5cfa893ea5f3
relation.isOrgUnitOfPublication 8eb24241-0d92-4baf-ae75-08f716d30801
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