The microbial utilization of levoglucosan has been explored in the recent decades (Zhuang and Zhang, 2002; Zhuang et al., 2001; Jarboe et al., 2011), but the literature concerning catabolism of cellobiosan, another component produced from thermochemical processing, is very scarce. This work focuses on the potential of cellobiosan to be metabolized by microbes isolated from soil as a sole carbon source. This will contribute to the study on the utilization of cellobiosan as an energy source during fermentation to produce useful products in the green chemicals industries. The work presented has demonstrated that cellobiosan is able to be utilized as a sole carbon source and also during co-fermentation with the other pyrolysis product, levoglucosan. The ability of novel strains to maximize the utilization of cellobiosan is supported by the usage of mineral medium to ensure that cellobiosan is the only carbon and energy source during fermentation.