Distribution of CRISPR Types in Fluoroquinolone-Resistant Campylobacter jejuni Isolates

dc.contributor.author Adiguzel, Mehmet
dc.contributor.author Goulart, Debora
dc.contributor.author Wu, Zuowei
dc.contributor.author Pang, Jinji
dc.contributor.author Cengiz, Seyda
dc.contributor.author Zhang, Qijing
dc.contributor.author Sahin, Orhan
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2021-04-01T17:28:15.000
dc.date.accessioned 2021-04-30T12:25:42Z
dc.date.available 2021-04-30T12:25:42Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2021
dc.date.issued 2021-03-16
dc.description.abstract <p>To aid development of phage therapy against Campylobacter, we investigated the distribution of the clustered regularly interspaced short palindromic repeats (CRISPR) systems in fluoroquinolone (FQ)-resistant Campylobacter jejuni. A total of 100 FQ-resistant C. jejuni strains from different sources were analyzed by PCR and DNA sequencing to determine resistance-conferring mutation in the gyrA gene and the presence of various CRISPR systems. All but one isolate harbored 1–5 point mutations in gyrA, and the most common mutation was the Thr86Ile change. Ninety-five isolates were positive with the CRISPR PCR, and spacer sequences were found in 86 of them. Among the 292 spacer sequences identified in this study, 204 shared 93–100% nucleotide homology to Campylobacter phage D10, 44 showed 100% homology to Campylobacter phage CP39, and 3 had 100% homology with Campylobacter phage CJIE4-5. The remaining 41 spacer sequences did not match with any phages in the database. Based on the results, it was inferred that the FQ-resistant C. jejuni isolates analyzed in this study were potentially resistant to Campylobacter phages D10, CP39, and CJIE4-5 as well as some unidentified phages. These phages should be excluded from cocktails of phages that may be utilized to treat FQ-resistant Campylobacter.</p>
dc.description.comments <p>This article is published as Adiguzel, Mehmet Cemal, Debora Brito Goulart, Zuowei Wu, Jinji Pang, Seyda Cengiz, Qijing Zhang, and Orhan Sahin. "Distribution of CRISPR Types in Fluoroquinolone-Resistant Campylobacter jejuni Isolates." <em>Pathogens</em> 10, no. 3 (2021): 345. DOI: <a href="https://doi.org/10.3390/pathogens10030345" target="_blank">10.3390/pathogens10030345</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/218/
dc.identifier.articleid 1217
dc.identifier.contextkey 22270534
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/218
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/105187
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/218/2021_ZhangQijing_DistributionCRISPR.pdf|||Fri Jan 14 22:39:12 UTC 2022
dc.source.uri 10.3390/pathogens10030345
dc.subject.disciplines Veterinary Infectious Diseases
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.keywords Campylobacter jejuni
dc.subject.keywords Cas9 gene
dc.subject.keywords CRISPR-Cas system
dc.subject.keywords fluoroquinolone-resistant bacteria
dc.title Distribution of CRISPR Types in Fluoroquinolone-Resistant Campylobacter jejuni Isolates
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 1c6a5dfc-c604-457f-85be-122910db782e
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
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