Cellular Distribution of Anionic Antimicrobial Peptide in Normal Lung and during Acute Pulmonary Inflammation

dc.contributor.author Gallup, Jack
dc.contributor.author Fales-Williams, Amanda
dc.contributor.author Brogden, Kim
dc.contributor.author Huffman, E.
dc.contributor.author Ackermann, Mark
dc.contributor.author Gallup, Jack
dc.contributor.author Ackermann, Mark
dc.contributor.department Veterinary Pathology
dc.date 2018-02-13T06:25:41.000
dc.date.accessioned 2020-07-07T05:15:52Z
dc.date.available 2020-07-07T05:15:52Z
dc.date.embargo 2013-02-26
dc.date.issued 2002-11-01
dc.description.abstract <p>Anionic peptides (APs) are small antimicrobial peptides present in human and ovine lung. In this study APs were also detected in bovine lung, and production of APs in lungs with acute inflammation induced by various stimuli was determined. The distribution and intensity of APs were determined by immunohistochemistry in lungs of 1) neonatal calves (1-3 days of age) inoculated with <em>Mannheimia (Pasteurella) haemolytica</em>, a known inducer of the bovine β-defensin lingual antimicrobial peptide (LAP) or pyrogen-free saline (PFS), and 2) growing calves (3 months of age) similarly inoculated with <em>M. haemolytica</em>, a lipopolysaccharide (LPS) from <em>M. haemolytica</em>, an LPS-associated protein from <em>M. haemolytica</em>, or PFS. APs were also detected by western blots with the same antibody in lungs of the calves above, as well as in calves inoculated with Pseudomonas aeruginosa, and an adult cow. Anionic peptide (AP) immunoreactivity was detected in bands (approximate weights) in the western blots of lung at 28-30 (strongest signal), 31, 45, and 52-60 kd regardless of inoculum. The adult cow lacked bands at 45 kd, but it had additional bands at 64 (inconsistently) and 35-38 kd. All these band sizes are consistent with those of the western blots of human and ovine lung. The cellular distribution of APs in lung of neonatal and growing cattle was similar to that in lung of human and sheep. In lungs with acute inflammation induced by live bacteria, LPS, or protein, AP distribution and intensity were similar to those in control (PFS-inoculated) lungs and slightly decreased in bronchioles. This work demonstrates that AP is present in lung of cattle and is thereby conserved among two ruminant species and man. Distribution and intensity of AP production are not enhanced by infection or acute inflammation and are decreased in bronchioles, which suggests that AP is not induced like β-defensins such as LAP, but, instead, is produced constitutively.</p>
dc.description.comments <p>This article is from <em>Veterinary Pathology </em>39, no. 6 (November 2002): 706–711, doi:<a href="http://dx.doi.org/10.1354/vp.39-6-706" target="_blank">10.1354/vp.39-6-706</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vpath_pubs/35/
dc.identifier.articleid 1038
dc.identifier.contextkey 3791096
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vpath_pubs/35
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92459
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vpath_pubs/35/2002_FalesWilliams_CellularDistributionAnionic.pdf|||Fri Jan 14 23:43:13 UTC 2022
dc.source.uri 10.1354/vp.39-6-706
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.keywords Anionic peptide
dc.subject.keywords Antimicrobial peptides
dc.subject.keywords Pneumonia
dc.subject.keywords Shipping fever
dc.title Cellular Distribution of Anionic Antimicrobial Peptide in Normal Lung and during Acute Pulmonary Inflammation
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isAuthorOfPublication 86c1ed73-b60d-48ce-8f35-449bc320a693
relation.isOrgUnitOfPublication cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f
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