Occurrence of Pathogenicity Island IAPEC-O1 Genes Among Escherichia coli Implicated in Avian Colibacillosis

dc.contributor.author Kariyawasam, Subhashinie
dc.contributor.author Johnson, Timothy
dc.contributor.author DebRoy, Chitrita
dc.contributor.author Nolan, Lisa
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-13T10:46:35.000
dc.date.accessioned 2020-07-07T05:15:11Z
dc.date.available 2020-07-07T05:15:11Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2006
dc.date.embargo 2013-05-08
dc.date.issued 2006-09-01
dc.description.abstract <p>Colibacillosis caused by avian pathogenic <em>Escherichia coli</em> (APEC) is a leading cause of economic loss to the poultry industry worldwide. The ability of APEC to cause disease is determined by certain virulence markers, some of which are located on pathogenicity islands (PAIs). We recently described one such PAI in an APEC O1:K1 strain (APEC-O1). This PAI, termed PAI I<sub>APEC-O1</sub>, carries the genes of the <em>pap</em> operon, a region similar to the <em>tia</em>invasion determinant of enterotoxigenic <em>E. coli</em>; <em>ireA</em>, a gene that encodes an iron-responsive element; and a novel 1.5-kb region, ORF 54. Here, the occurrence of six selected loci of PAI I<sub>APEC-O1</sub> (<em>papA, papC, papG, ireA, tia,</em> and ORF 54) among APEC and fecal <em>E. coli</em>strains from apparently healthy chickens (avian commensal <em>E. coli</em>) was determined using polymerase chain reaction (PCR) techniques. None of the commensal <em>E. coli</em> was positive for all six traits, whereas 7.2% of the APEC isolates were positive for all the traits. Although there was no significant difference in the occurrence of ORF 54 among APEC and commensal <em>E. coli</em>, <em>tia, ireA, papC</em>, and <em>papG</em> genes were predominantly present in APEC rather than in avian commensal <em>E. coli</em>. <em>papA</em> was detected in only 6.3% of APEC, perhaps because of the presence of allelic variants of the gene. Additionally, the presence of all six traits was tested with PCR in APEC isolates collected in the 1980s, and these results were compared with those obtained with the APEC isolated in the 1990s. There was no significant difference in the occurrence of <em>tia, ireA, papC, papG</em>, and ORF 54 between APEC isolates collected during the different decades. However, <em>papA</em> was more frequently present in APEC from the 1980s than it was in APEC from the 1990s. Phylogenetic group of an isolate did not correlate with pathogenicity or the presence of PAI traits, except that more APEC of the low-pathogenicity group belonged to the phylogenetic group B1. However, PAI traits occurred more frequently in isolates belonging to the intermediate- and high-pathogenicity groups than in isolates of low pathogenicity.</p>
dc.description.comments <p>This article is from <em>Avian Diseases</em> 50, no. 3 (2006): 405–410, doi:<a href="http://dx.doi.org.proxy.lib.iastate.edu/10.1637/7462-102705R.1" target="_blank">10.1637/7462-102705R.1</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/41/
dc.identifier.articleid 1044
dc.identifier.contextkey 4117486
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/41
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92346
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/41/2006_KariyawasamS_OccurrencePathogenicityIsland.pdf|||Sat Jan 15 00:09:11 UTC 2022
dc.source.uri 10.1637/7462-102705R.1
dc.subject.disciplines Genomics
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords avian pathogenic Escherichia coli
dc.subject.keywords extraintestinal pathogenic E. coli
dc.subject.keywords pathogenicity island
dc.subject.keywords pap operon
dc.subject.keywords tia gene
dc.subject.keywords ireA gene
dc.subject.keywords phylogenetic typing
dc.subject.keywords polymerase chain reaction
dc.subject.keywords emerging pathogen
dc.title Occurrence of Pathogenicity Island IAPEC-O1 Genes Among Escherichia coli Implicated in Avian Colibacillosis
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 9e7506b4-e945-47cf-9195-e814dac6c9fd
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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