Detection of porcine epidemic diarrhea virus (PEDV) IgG and IgA in muscle tissue exudate (“meat juice”) specimens

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2018-12-18
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Poonsuk, Korakrit
Cheng, Ting-Yu
Ji, Ju
Zimmerman, Jeffrey
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Springer Nature
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Giménez-Lirola, Luis
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Veterinary Diagnostic and Production Animal Medicine
The mission of VDPAM is to educate current and future food animal veterinarians, population medicine scientists and stakeholders by increasing our understanding of issues that impact the health, productivity and well-being of food and fiber producing animals; developing innovative solutions for animal health and food safety; and providing the highest quality, most comprehensive clinical practice and diagnostic services. Our department is made up of highly trained specialists who span a wide range of veterinary disciplines and species interests. We have faculty of all ranks with expertise in diagnostics, medicine, surgery, pathology, microbiology, epidemiology, public health, and production medicine. Most have earned certification from specialty boards. Dozens of additional scientists and laboratory technicians support the research and service components of our department.
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Statistics
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The diagnostic performance of porcine epidemic diarrhea virus (PEDV) IgG and IgA ELISAs was evaluated using paired serum and meat juice samples collected from PEDV-negative (n = 50) and PEDV-inoculated pigs (n = 87). Serum samples were tested by PEDV (IgG, IgA) ELISAs using a procedure performed routinely at the Iowa State University-Veterinary Diagnostic Laboratory (ISU-VDL). Serum samples were tested using PEDV serum IgG and IgA ELISA procedures as routinely performed at the Iowa State University-Veterinary Diagnostic Laboratory (ISU-VDL). Serum samples were diluted 1:50 and conjugate concentrations were 1/20,000 for IgG and 1/3000 for IgA. Meat juice samples were tested using the serum PEDV IgG and IgA ELISAs, with modifications, i.e., meat juice samples were diluted 1:25 and conjugate concentrations were 1/40,000 for IgG and 1/10,000 for IgA. Receiver operator characteristic (ROC) curve analyses were used to estimate diagnostic sensitivities and specificities over a range of sample-to-positive (S/P) cutoffs. Consistent with previous reports, this study showed that the PEDV IgG and IgA meat juice ELISAs provided excellent diagnostic performance and suggest that meat juice recovered from samples collected at slaughter could be used in routine PEDV surveillance.
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This article is published as Poonsuk, Korakrit, Ting-Yu Cheng, Ju Ji, Jeffrey Zimmerman, and Luis Giménez-Lirola. "Detection of porcine epidemic diarrhea virus (PEDV) IgG and IgA in muscle tissue exudate (“meat juice”) specimens." Porcine Health Management 4, no. 1 (2018): 1-5. DOI: 10.1186/s40813-018-0107-4. Copyright 2018 The Author(s). Attribution 4.0 International (CC BY 4.0). Posted with permission.
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