Chemiluminescence by Listeria monocytogenes

dc.contributor.author Roth, James
dc.contributor.author Kaeberle, Merlin
dc.contributor.author Roth, James
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-04-12T18:44:40.000
dc.date.accessioned 2020-07-07T05:14:51Z
dc.date.available 2020-07-07T05:14:51Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 1980
dc.date.issued 1980-11-01
dc.description.abstract <p>Listeria monocytogenes cells suspended in brain heart infusion broth or in carbonated saline solution emitted light (chemiluminescence) that could be detected by a liquid scintillation spectrometer. This chemiluminescence was inhibited by superoxide dismutase and catalase but not by the hydroxyl radical scavengers mannitol and benzoate; it was also dependent upon and proportional to the carbonate ion concentration in the medium. Organisms suspended in carbonated saline solution which had ceased to chemiluminesce immediately began to chemiluminesce again when acetaldehyde was added but not when glucose, sucrose, or xanthine was added. Acetaldehyde-induced chemiluminescence was inhibited by suproxide dismutase and catalase but not by allopurinol. Our data indicate that the superoxide anion, hydrogen peroxide, and the carbonate ion are involved in chemiluminescence by L. monocytogenes. Chemiluminescence is apparently initiated by the extracellular generation of superoxide anon by this organism. The mechanism for the production of the superoxide anion is not known, but xanthine oxidase does not appear to be involved.</p>
dc.description.comments <p>This article is published as Roth, J.A., and M.L. Kaeberle. 1980. Chemiluminescence by <em>Listeria monocytogenes.</em> J Bacteriol, 144:752-757. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/184/
dc.identifier.articleid 1198
dc.identifier.contextkey 11952492
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/184
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92292
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/184/1980_Roth_ChemiluminescenceListeria.pdf|||Fri Jan 14 21:41:29 UTC 2022
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Chemiluminescence by Listeria monocytogenes
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 909dd0b2-ec0a-41e2-b4e0-9e5ff76b7622
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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