Analysis of Restriction Fragment Length Polymorphisms of the Major Histocompatibility Complex of 15I5-B-Congenic Chicken Lines

dc.contributor.author Lamont, Susan
dc.contributor.author Gerndt, Bradley
dc.contributor.author Lamont, Susan
dc.contributor.author Warner, C. M.
dc.contributor.author Bacon, Larry
dc.contributor.department Animal Science
dc.date 2018-02-17T06:22:00.000
dc.date.accessioned 2020-06-29T23:39:02Z
dc.date.available 2020-06-29T23:39:02Z
dc.date.issued 1990
dc.description.abstract <p>Eight 15I<sub>5</sub> <em>B</em>-congenic White Leghorn chicken lines, containing haplotypes<em>B</em> <sup>2</sup> <em>, B</em> <sup>5</sup> <em>, B</em> <sup>12</sup>, <em>B</em> <sup>13</sup>, <em>B</em> <sup>15</sup> <em>, B</em> <sup>19</sup> <em>,</em> and <em>B</em> <sup>21</sup>, were subjected to molecular genotyping with chicken <em>B</em>-<em>F</em> (Class I) and <em>B-L</em> (Class II) major histocompatibility complex (MHC) probes. Genomic DNA was digested with restriction enzymes, hybridized with a Class I or Class II probe, and analyzed for restriction fragment length polymorphisms. Digestion with<em>Hind</em>III or <em>Eco</em>RI yielded no <em>B-L</em> polymorphisms. Digestion with <em>Pvu</em>II or <em>Bgl</em>II and hybridization with a <em>B-L</em> or <em>B-F</em> probe produced polymorphisms that were shared between several haplotypes, although the haplotypes with similar patterns were clustered differently between Class I and Class II probes. The genetic variation seen for <em>B-L</em> and <em>B-F</em> probe hybridization of<em>Pvu</em>II digests was much less than that previously demonstrated for <em>B-G</em>probing of <em>Pvu</em>II digests of the same lines. Description of MHC Class I and II restriction patterns of the well-characterized 15I<sub>5</sub> <em>B</em>-congenic lines will aid in identification of genes important in disease resistance.</p>
dc.description.comments <p>This article is from <em>Poultry Science</em> 69 (1990): 1195, doi:<a href="http://dx.doi.org/10.3382/ps.0691195" target="_blank">10.3382/ps.0691195</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/210/
dc.identifier.articleid 1208
dc.identifier.contextkey 7855990
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/210
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/9617
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/210/1990_Lamont_AnalysisRestriction.pdf|||Fri Jan 14 22:33:49 UTC 2022
dc.source.uri 10.3382/ps.0691195
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Poultry or Avian Science
dc.subject.keywords major histocompatibility complex
dc.subject.keywords restriction fragment length polymorphisms
dc.subject.keywords B-congenic lines
dc.subject.keywords chickens
dc.title Analysis of Restriction Fragment Length Polymorphisms of the Major Histocompatibility Complex of 15I5-B-Congenic Chicken Lines
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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