Detection of RNA from a Novel West Nile-like Virus and High Prevalence of an Insect-specific Flavivirus in Mosquitoes in the Yucatan Peninsula of Mexico

dc.contributor.author Farfan-Ale, Jose
dc.contributor.author Lorono-Pino, Maria
dc.contributor.author Garcia-Rejon, Julian
dc.contributor.author Hovav, Einat
dc.contributor.author Powers, Ann
dc.contributor.author Lin, Ming
dc.contributor.author Dorman, Karin
dc.contributor.author Platt, Kenneth
dc.contributor.author Bartholomay, Lyric
dc.contributor.author Soto, Victor
dc.contributor.author Beaty, Barry
dc.contributor.author Lanciotti, Robert
dc.contributor.author Blitvich, Bradley
dc.contributor.department Entomology
dc.date 2018-02-14T14:45:14.000
dc.date.accessioned 2020-06-30T02:22:01Z
dc.date.available 2020-06-30T02:22:01Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 2009
dc.date.embargo 2014-09-10
dc.date.issued 2009-01-01
dc.description.abstract <p>As part of our ongoing surveillance efforts for West Nile virus (WNV) in the Yucatan Peninsula of Mexico, 96,687 mosquitoes collected from January through December 2007 were assayed by virus isolation in mammalian cells. Three mosquito pools caused cytopathic effect. Two isolates were orthobunyaviruses (Cache Valley virus and Kairi virus) and the identity of the third infectious agent was not determined. A subset of mosquitoes was also tested by reverse transcription-polymerase chain reaction (RT-PCR) using WNV-, flavivirus-, alphavirus-, and orthobunyavirus-specific primers. A total of 7,009 Culex quinquefasciatus in 210 pools were analyzed. Flavivirus RNA was detected in 146 (70%) pools, and all PCR products were sequenced. The nucleotide sequence of one PCR product was most closely related (71-73% identity) with homologous regions of several other flaviviruses, including WNV, St. Louis encephalitis virus, and Ilheus virus. These data suggest that a novel flavivirus (tentatively named T'Ho virus) is present in Mexico. The other 145 PCR products correspond to Culex flavivirus, an insect-specific flavivirus first isolated in Japan in 2003. Culex flavivirus was isolated in mosquito cells from approximately one in four homogenates tested. The genomic sequence of one isolate was determined. Surprisingly, heterogeneous sequences were identified at the distal end of the 5' untranslated region.</p>
dc.description.comments <p>This is an author's manuscript of an article from <em>The American journal of tropical medicine and hygiene</em> 80 (2008): 85.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ent_pubs/154/
dc.identifier.articleid 1152
dc.identifier.contextkey 6101000
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ent_pubs/154
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/23757
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ent_pubs/154/2008_Bartholomay_DetectionRNA.pdf|||Fri Jan 14 20:40:19 UTC 2022
dc.subject.disciplines Entomology
dc.subject.disciplines Molecular Genetics
dc.subject.disciplines Virology
dc.subject.disciplines Virus Diseases
dc.title Detection of RNA from a Novel West Nile-like Virus and High Prevalence of an Insect-specific Flavivirus in Mosquitoes in the Yucatan Peninsula of Mexico
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isOrgUnitOfPublication f47c8cad-50be-4fb0-8870-902ff536748c
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