Co-Expression Networks Reveal Potential Regulatory Roles of miRNAs in Fatty Acid Composition of Nelore Cattle de Oliveira, Priscila Coutinho, Luiz Reecy, James Cesar, Aline da Silva Diniz, Wellison de Souza, Marcela Andrade, Bruno Koltes, James Mourão, Gerson Zerlotini, Adhemar Reecy, James Regitano, Luciana
dc.contributor.department Animal Science 2019-09-13T02:10:07.000 2020-06-29T23:41:04Z 2020-06-29T23:41:04Z Tue Jan 01 00:00:00 UTC 2019 2019-07-11
dc.description.abstract <p>Fatty acid (FA) content affects the sensorial and nutritional value of meat and plays a significant role in biological processes such as adipogenesis and immune response. It is well known that, in beef, the main FAs associated with these biological processes are oleic acid (C18:1 cis9, OA) and conjugated linoleic acid (CLA-c9t11), which may have beneficial effects on metabolic diseases such as type 2 diabetes and obesity. Here, we performed differential expression and co-expression analyses, weighted gene co-expression network analysis (WGCNA) and partial correlation with information theory (PCIT), to uncover the complex interactions between miRNAs and mRNAs expressed in skeletal muscle associated with FA content. miRNA and mRNA expression data were obtained from skeletal muscle of Nelore cattle that had extreme genomic breeding values for OA and CLA. Insulin and MAPK signaling pathways were identified by WGCNA as central pathways associated with both of these fatty acids. Co-expression network analysis identified bta-miR-33a/b, bta-miR-100, bta-miR-204, bta-miR-365-5p, bta-miR-660, bta-miR-411a, bta-miR-136, bta-miR-30-5p, bta-miR-146b, bta-let-7a-5p, bta-let-7f, bta-let-7, bta-miR 339, bta-miR-10b, bta-miR 486, and the genes <em>ACTA1</em> and <em>ALDOA</em> as potential regulators of fatty acid synthesis. This study provides evidence and insights into the molecular mechanisms and potential target genes involved in fatty acid content differences in Nelore beef cattle, revealing new candidate pathways of phenotype modulation that could positively benefit beef production and human consumption.</p>
dc.description.comments <p>This article is published as De Oliveira, Priscila SN, Luiz Lehmann Coutinho, Aline Silva Mello Cesar, Wellison Jarles Da Silva Diniz, Marcela Maria De Souza, Bruno Gabriel Nascimento Andrade, James E. Koltes et al. "Co-expression networks reveals potential regulatory roles of miRNAs in fatty acids composition of Nelore cattle." <em>Frontiers in Genetics</em> 10 (2019): 651. doi: <a href="">10.3389/fgene.2019.00651</a>. </p>
dc.format.mimetype application/pdf
dc.identifier archive/
dc.identifier.articleid 1469
dc.identifier.contextkey 14925256
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/469
dc.language.iso en
dc.source.bitstream archive/|||Sat Jan 15 00:24:10 UTC 2022
dc.source.uri 10.3389/fgene.2019.00651
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Beef Science
dc.subject.disciplines Genetics
dc.subject.disciplines Molecular Genetics
dc.subject.keywords Bos indicus
dc.subject.keywords conjugated linoleic acid
dc.subject.keywords integrative genomics
dc.subject.keywords mRNA
dc.subject.keywords miRNA
dc.subject.keywords oleic acid
dc.title Co-Expression Networks Reveal Potential Regulatory Roles of miRNAs in Fatty Acid Composition of Nelore Cattle
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication fb994cd9-94d5-4370-94ab-f33934c4cd6f
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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