Two different aeration plates are used to study their effect on gas holdup and flow regime transition in fiber suspensions. Two gas distributors with different open areas (A = 0.57% and 2.14%) and the same orifice diameter (do = 1 mm) are used, and experiments are performed using three different Rayon fiber lengths (L = 3, 6, and 12 mm) over a range of superficial gas velocities (Ug ≤ 18 cm/s) and a range of fiber mass fractions (0 ≤ C ≤ 1.8%) in a 15.24 cm diameter semi-batch bubble column. Experimental results show that the distributor with A = 2.14% tends to produce lower gas holdup than the one with A = 0.57% for both air-water systems and fiber slurries. However, the effect of distributor open area on gas holdup diminishes at high fiber mass fractions (C ≤ 1.2%). Both distributors generate homogeneous, transitional, and heterogeneous flow regimes over the range of superficial gas velocities for air-water and low fiber mass fraction suspensions. However, the distributor with A = 2.14% enhances the flow regime transition, i.e., the superficial gas velocity at which the transitional flow regime appears is lower. Additionally, the fiber mass fraction at which purely heterogeneous flow is observed is lower when A = 2.14%.