Enzyme immunoassay for the rapid detection of Escherichia coli O157
Jung, Suk Chan
Kim, Jong Man
An, Soo Hwan
Kim, Bong Hwan
An enzyme immunoassay(EIA) to detect Escherichia(E.) coli 0157 in pork was developed by using a sandwich-type assay on the 96-well microplates. All E. coli O157 strains and Citrobacter amalonaticus reacted strongly, however 29 E. coli serotypes and 15 non-E. coli bacterial strains showed negative in E. coli O157 EIA. E. coli 0157 in pork could be detected with in 13 h including 10 h in enrichment broth and 3 h in EIA. As few as 1.8 CFU of E. coli O157 per g of pork could be detected after enrichment, whereas above 1.8 >. 1 o5 CFU of E. coli O157 per g of pork could be detected without enrichment. The E. coli 0157 EIA was a sensitive, easy-to-perform and efficient method for the screening of E. coliO 157 in pork.