The JIL-1 kinase interacts with lamin Dm0 and regulates nuclear lamina morphology of Drosophila nurse cells

dc.contributor.author Bao, Xiaomin
dc.contributor.author Zhang, Weiguo
dc.contributor.author Johansen, Kristen
dc.contributor.author Krencik, Robert
dc.contributor.author Deng, Huai
dc.contributor.author Wang, Yanming
dc.contributor.author Girton, Jack
dc.contributor.author Johansen, Jorgen
dc.contributor.author Johansen, Kristen
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.date 2019-07-09T03:46:14.000
dc.date.accessioned 2020-06-29T23:46:36Z
dc.date.available 2020-06-29T23:46:36Z
dc.date.copyright Sat Jan 01 00:00:00 UTC 2005
dc.date.issued 2005-01-01
dc.description.abstract <p>We have used a yeast two-hybrid screen to identify lamin Dm0 as an interaction partner for the nuclear JIL-1 kinase. This molecular interaction was confirmed by GST-fusion protein pull-down assays and by co-immunoprecipitation experiments. Using deletion construct analysis we show that a predicted globular domain of the basic region of the COOH-terminal domain of JIL-1 was sufficient for mediating the molecular interactions with lamin Dm0. A reciprocal analysis with truncated lamin Dm0 constructs showed that the interaction with JIL-1 required sequences in the tail domain of lamin Dm0 that include the Ig-like fold. Further support for a molecular interaction between JIL-1 and lamin Dm0 in vivo was provided by genetic interaction assays. We show that nuclear positioning and lamina morphology were abnormal in <em>JIL-1</em> mutant egg chambers. The most common phenotypes observed were abnormal nurse cell nuclear lamina protrusions through the ring canals near the oocyte, as well as dispersed and mislocalized lamin throughout the egg chamber. These phenotypes were completely rescued by a full-length <em>JIL-1</em> transgenic construct. Thus, our results suggest that the JIL-1 kinase is required to maintain nuclear morphology and integrity of nurse cells during oogenesis and that this function may be linked to molecular interactions with lamin Dm0.</p>
dc.description.comments <p>This article is published as Bao, Xiaomin, Weiguo Zhang, Robert Krencik, Huai Deng, Yanming Wang, Jack Girton, Jørgen Johansen, and Kristen M. Johansen. "The JIL-1 kinase interacts with lamin Dm0 and regulates nuclear lamina morphology of Drosophila nurse cells." <em>Journal of cell science</em> 118, no. 21 (2005): 5079-5087. doi: <a href="http://dx.doi.org/10.1242/jcs.02611" target="_blank">10.1242/jcs.02611</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/bbmb_ag_pubs/226/
dc.identifier.articleid 1233
dc.identifier.contextkey 14517335
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath bbmb_ag_pubs/226
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/10695
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/bbmb_ag_pubs/226/2005_Johansen_KinaseInteracts.pdf|||Fri Jan 14 22:44:00 UTC 2022
dc.source.uri 10.1242/jcs.02611
dc.subject.disciplines Biochemistry
dc.subject.disciplines Biophysics
dc.subject.disciplines Genetics
dc.subject.disciplines Molecular Biology
dc.subject.disciplines Structural Biology
dc.subject.keywords Lamin
dc.subject.keywords Oogenesis
dc.subject.keywords Nuclear lamina
dc.subject.keywords JIL-1
dc.subject.keywords Drosophila
dc.title The JIL-1 kinase interacts with lamin Dm0 and regulates nuclear lamina morphology of Drosophila nurse cells
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 3829d759-f8ec-4502-a63a-1655d3b70ef5
relation.isOrgUnitOfPublication c70f85ae-e0cd-4dce-96b5-4388aac08b3f
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