Interaction-Dependent Gene Expression in Mla-Specified Response to Barley Powdery Mildew

dc.contributor.author Caldo, Rico
dc.contributor.author Nettleton, Dan
dc.contributor.author Wise, Roger
dc.contributor.author Nettleton, Dan
dc.contributor.department Plant Pathology and Microbiology
dc.contributor.department Statistics
dc.date 2019-08-25T17:18:09.000
dc.date.accessioned 2020-07-02T06:57:16Z
dc.date.available 2020-07-02T06:57:16Z
dc.date.issued 2004-09-01
dc.description.abstract <p>Plant recognition of pathogen-derived molecules influences attack and counterattack strategies that affect the outcome of host–microbe interactions. To ascertain the global framework of host gene expression during biotrophic pathogen invasion, we analyzed in parallel the mRNA abundance of 22,792 host genes throughout 36 (genotype × pathogen × time) interactions between barley (<em>Hordeum vulgare</em>) and <em>Blumeria graminis</em> f. sp <em>hordei</em> (<em>Bgh</em>), the causal agent of powdery mildew disease. A split-split-plot design was used to investigate near-isogenic barley lines with introgressed <em>Mla6</em>, <em>Mla13</em>, and <em>Mla1</em> coiled-coil, nucleotide binding site, Leu-rich repeat resistance alleles challenged with <em>Bgh</em> isolates 5874 (<em>AvrMla6</em> and <em>AvrMla1</em>) and K1 (<em>AvrMla13</em> and <em>AvrMla1</em>). A linear mixed model analysis was employed to identify genes with significant differential expression (P value < 0.0001) in incompatible and compatible barley-<em>Bgh</em> interactions across six time points after pathogen challenge. Twenty-two host genes, of which five were of unknown function, exhibited highly similar patterns of upregulation among all incompatible and compatible interactions up to 16 h after inoculation (hai), coinciding with germination of <em>Bgh</em> conidiospores and formation of appressoria. By contrast, significant divergent expression was observed from 16 to 32 hai, during membrane-to-membrane contact between fungal haustoria and host epidermal cells, with notable suppression of most transcripts identified as differentially expressed in compatible interactions. These findings provide a link between the recognition of general and specific pathogen-associated molecules in gene-for-gene specified resistance and support the hypothesis that host-specific resistance evolved from the recognition and prevention of the pathogen's suppression of plant basal defense.</p>
dc.description.comments <p>This article is published as Caldo, Rico A., Dan Nettleton, and Roger P. Wise. "Interaction-dependent gene expression in Mla-specified response to barley powdery mildew." <em>The Plant Cell</em> 16, no. 9 (2004): 2514-2528. doi: <a href="https://doi.org/10.1105/tpc.104.023382" target="_blank">10.1105/tpc.104.023382</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/stat_las_pubs/220/
dc.identifier.articleid 1222
dc.identifier.contextkey 14884130
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath stat_las_pubs/220
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/90533
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/stat_las_pubs/220/2004_Nettleton_InteractionDependent.pdf|||Fri Jan 14 22:41:52 UTC 2022
dc.source.uri 10.1105/tpc.104.023382
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Molecular Genetics
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.disciplines Plant Pathology
dc.subject.disciplines Statistical Models
dc.title Interaction-Dependent Gene Expression in Mla-Specified Response to Barley Powdery Mildew
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 7d86677d-f28f-4ab1-8cf7-70378992f75b
relation.isOrgUnitOfPublication a26b5928-54bb-4a0b-a973-95d649d1ad83
relation.isOrgUnitOfPublication 264904d9-9e66-4169-8e11-034e537ddbca
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