Tuning Surface Functionalization Collagen Gel Thickness to Regulate Cancer Cell Migration

dc.contributor.author Bartlett, Michael
dc.contributor.author Unnikandam Veettil, Shalini
dc.contributor.author Van Bruggen, Shawn
dc.contributor.author Hwang, Doh-Gyu
dc.contributor.author Schneider, Ian
dc.contributor.author Bartlett, Michael
dc.contributor.author Schneider, Ian
dc.contributor.department Materials Science and Engineering
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Chemical and Biological Engineering
dc.date 2019-05-13T02:13:11.000
dc.date.accessioned 2020-06-30T01:09:59Z
dc.date.available 2020-06-30T01:09:59Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2019
dc.date.embargo 2021-03-16
dc.date.issued 2019-07-01
dc.description.abstract <p>Cancer cells have a tremendous ability to sense and respond to extracellular matrix (ECM) stiffness, modulating invasion. The magnitude of the sensed stiffness can either promote or inhibit the migration of cancer cells out of the primary tumor into surrounding tissue. Work has been done on examining the role of stiffness in tuning cancer cell migration by controlling elastic modulus in the bulk. However, a powerful and complementary approach for controlling stiffness is to leverage interactions between stiff-soft (e.g. glass-hydrogel) interfaces. Unfortunately, most work in this area probes cells in 2D environments. Of the reports that probe 3D environments, none have assessed the role of mechanical linkage to the interface as a potential handle in controlling local stiffness and cell behavior. In this paper, we examine the migration of cancer cells embedded in a collagen fiber network between two flat plates. We examine the role of both surface attachment of the collagen network to the stiff interface as well as thickness (50-540 μm) of the collagen gel in driving collagen organization, cell morphology and cell migration. We find that surface attachment and thickness do not operate overlapping mechanisms, because they elicit different cell responses. While thickness and surface chemistry appear to control morphology, only thickness regulates collagen organization and cell migration speed. This suggests that surface attachment and thickness of the collagen gel control cell behavior through both collagen structure and local stiffness in confined fiber-forming networks.</p>
dc.description.comments <p>This is a manuscript of an article published as Unnikandam Veettil, Shalini R., Shawn M. Van Bruggen, Doh-Gyu Hwang, Michael D. Bartlett, and Ian C. Schneider. "Tuning Surface Functionalization Collagen Gel Thickness to Regulate Cancer Cell Migration." <em>Colloids and Surfaces B: Biointerfaces</em> 179 (2019): 37-47. DOI: <a href="http://dx.doi.org/10.1016/j.colsurfb.2019.03.031" target="_blank">10.1016/j.colsurfb.2019.03.031</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/cbe_pubs/368/
dc.identifier.articleid 1369
dc.identifier.contextkey 14138451
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath cbe_pubs/368
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/13470
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/cbe_pubs/368/0-2019_SchneiderIan_TuningSurface_Suppl.pdf|||Fri Jan 14 23:48:36 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/cbe_pubs/368/2019_SchneiderIan_TuningSurface.pdf|||Fri Jan 14 23:48:37 UTC 2022
dc.source.uri 10.1016/j.colsurfb.2019.03.031
dc.subject.disciplines Biochemical and Biomolecular Engineering
dc.subject.disciplines Biomechanical Engineering
dc.subject.disciplines Cancer Biology
dc.subject.disciplines Membrane Science
dc.subject.keywords Interface
dc.subject.keywords collagen
dc.subject.keywords adhesion
dc.subject.keywords morphology
dc.subject.keywords MDA-MB-231
dc.subject.keywords migration
dc.subject.keywords durotaxis
dc.subject.keywords mechanotaxis
dc.supplemental.bitstream 2019_SchneiderIan_TuningSurface_Suppl.pdf
dc.title Tuning Surface Functionalization Collagen Gel Thickness to Regulate Cancer Cell Migration
dc.type article
dc.type.genre article
dspace.entity.type Publication
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