Functions of Connexin 43.4 in Relation to Cell Adhesion and Beta-Catenin Localization

Abstract

I have analyzed several mutants in the connexin43.4 (cx43.4) gene and its relation to Kupffer’s vesicle (KV) formation within developing zebrafish embryos. Attempting to determine whether cx43.4 mutants affect cell adhesion, tight junction formation, and betacatenin localization. Our results over the course of this semester have indicated a possible affect of the cx43.4 mutants on beta-catenin localization. Further experiments will determine how cx43.4 contributes to early axis formation and formation of a luminal space. These are fundamental processes during development that position the internal organs and guide morphogenesis. The basic understanding of these processes has implications for strategies for biomedicine.