Transplantation of BDNF-Secreting Mesenchymal Stem Cells Provides Neuroprotection in Chronically Hypertensive Rat Eyes

dc.contributor.author Harper, Matthew
dc.contributor.author Grozdanic, Sinisa
dc.contributor.author Blits, Bas
dc.contributor.author Kuehn, Markus
dc.contributor.author Zamzow, Daniel
dc.contributor.author Sakaguchi, Donald
dc.contributor.author Buss, Janice
dc.contributor.author Kardon, Randy
dc.contributor.author Sakaguchi, Donald
dc.contributor.department Veterinary Clinical Sciences
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.contributor.department Neuroscience
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2018-02-18T11:55:49.000
dc.date.accessioned 2020-06-30T04:01:23Z
dc.date.available 2020-06-30T04:01:23Z
dc.date.issued 2011-06-01
dc.description.abstract <p>Purpose.: To evaluate the ability of mesenchymal stem cells (MSCs) engineered to produce and secrete brain-derived neurotrophic factor (BDNF) to protect retinal function and structure after intravitreal transplantation in a rat model of chronic ocular hypertension (COH).</p> <p>Methods.: COH was induced by laser cauterization of trabecular meshwork and episcleral veins in rat eyes. COH eyes received an intravitreal transplant of MSCs engineered to express BDNF and green fluorescent protein (BDNF-MSCs) or just GFP (GFP-MSCs). Computerized pupillometry and electroretinography (ERG) were performed to assess optic nerve and retinal function. Quantification of optic nerve damage was performed by counting retinal ganglion cells (RGCs) and evaluating optic nerve cross-sections.</p> <p>Results.: After transplantation into COH eyes, BDNF-MSCs preserved significantly more retina and optic nerve function than GFP-MSC–treated eyes when pupil light reflex (PLR) and ERG function were evaluated. PLR analysis showed significantly better function (<em>P</em> = 0.03) in BDNF-MSC–treated eyes (operated/control ratio = 63.00% ± 11.39%) than GFP-MSC–treated eyes (operated/control ratio = 31.81% ± 9.63%) at 42 days after surgery. The BDNF-MSC–transplanted eyes also displayed a greater level of RGC preservation than eyes that received the GFP-MSCs only (RGC cell counts: BDNF-MSC–treated COH eyes, 112.2 ± 19.39 cells/section; GFP-MSC–treated COH eyes, 52.21 ± 11.54 cells/section; <em>P</em> = 0.01).</p> <p>Conclusions.: The authors have demonstrated that lentiviral-transduced BDNF-producing MSCs can survive in eyes with chronic hypertension and can provide retina and optic nerve functional and structural protection. Transplantation of BDNF-producing stem cells may be a viable treatment strategy for glaucoma.</p>
dc.description.comments <p>This article is from <em>Investigative Ophthalmology & Visual Science </em>52 (2011): 4506, doi: <a href="http://dx.doi.org/10.1167/iovs.11-7346" target="_blank">10.1167/iovs.11-7346</a>.</p>
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dc.identifier archive/lib.dr.iastate.edu/gdcb_las_pubs/144/
dc.identifier.articleid 1146
dc.identifier.contextkey 10201765
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/144
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37811
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/gdcb_las_pubs/144/2011_Sakaguchi_TransplantationSecreting.pdf|||Fri Jan 14 20:19:41 UTC 2022
dc.source.uri 10.1167/iovs.11-7346
dc.subject.disciplines Biochemistry
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Comparative and Laboratory Animal Medicine
dc.subject.disciplines Molecular Biology
dc.subject.disciplines Structural Biology
dc.title Transplantation of BDNF-Secreting Mesenchymal Stem Cells Provides Neuroprotection in Chronically Hypertensive Rat Eyes
dc.type article
dc.type.genre article
dspace.entity.type Publication
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