The maize brown midrib4 (bm4) gene encodes a functional folylpolyglutamate synthase

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dc.contributor.author Li, Li
dc.contributor.author Nettleton, Dan
dc.contributor.author Hill-Skinner, Sarah
dc.contributor.author Liu, Sanzhen
dc.contributor.author Beuchle, Danielle
dc.contributor.author Tang, Ho Man
dc.contributor.author Yeh, Cheng-Ting
dc.contributor.author Nettleton, Dan
dc.contributor.author Schnable, Patrick
dc.contributor.department Statistics
dc.contributor.department Agronomy
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Center for Plant Genomics
dc.date 2019-08-22T08:02:25.000
dc.date.accessioned 2020-07-02T06:57:06Z
dc.date.available 2020-07-02T06:57:06Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.issued 2015-02-01
dc.description.abstract <p>Mutations in the <em>brown midrib4 (bm4)</em> gene affect the accumulation and composition of lignin in maize. Fine‐mapping analysis of <em>bm4</em> narrowed the candidate region to an approximately 105 kb interval on chromosome 9 containing six genes. Only one of these six genes, GRMZM2G393334, showed decreased expression in mutants. At least four of 10 <em>Mu</em>‐induced <em>bm4</em> mutant alleles contain a <em>Mu</em> insertion in the GRMZM2G393334 gene. Based on these results, we concluded that GRMZM2G393334 is the <em>bm4</em> gene. GRMZM2G393334 encodes a putative folylpolyglutamate synthase (FPGS), which functions in one‐carbon (C1) metabolism to polyglutamylate substrates of folate‐dependent enzymes. Yeast complementation experiments demonstrated that expression of the maize <em>bm4</em> gene in FPGS‐deficient <em>met7</em> yeast is able to rescue the yeast mutant phenotype, thus demonstrating that <em>bm4</em> encodes a functional FPGS. Consistent with earlier studies, <em>bm4</em> mutants exhibit a modest decrease in lignin concentration and an overall increase in the S:G lignin ratio relative to wild‐type. Orthologs of <em>bm4</em> include at least one paralogous gene in maize and various homologs in other grasses and dicots. Discovery of the gene underlying the <em>bm4</em> maize phenotype illustrates a role for FPGS in lignin biosynthesis.</p>
dc.description.comments <p>This article is published as Li, Li, Sarah Hill‐Skinner, Sanzhen Liu, Danielle Beuchle, Ho Man Tang, Cheng‐Ting Yeh, Dan Nettleton, and Patrick S. Schnable. "The maize brown midrib4 (bm4) gene encodes a functional folylpolyglutamate synthase." <em>The Plant Journal</em> 81, no. 3 (2015): 493-504. doi: <a href="https://doi.org/10.1111/tpj.12745">10.1111/tpj.12745</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/stat_las_pubs/194/
dc.identifier.articleid 1192
dc.identifier.contextkey 14813921
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath stat_las_pubs/194
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/90503
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/stat_las_pubs/194/2015_Nettleton_MaizeBrown.pdf|||Fri Jan 14 21:56:23 UTC 2022
dc.source.uri 10.1111/tpj.12745
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.keywords brown midrib
dc.subject.keywords lignin
dc.subject.keywords folylpolyglutamate synthase
dc.subject.keywords folylpolyglutamate synthase
dc.subject.keywords Zea mays
dc.subject.keywords methylenetetrahydrofolate reductase
dc.title The maize brown midrib4 (bm4) gene encodes a functional folylpolyglutamate synthase
dc.type article
dc.type.genre article
dspace.entity.type Publication
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