Differential immunological response detected in mRNA expression profiles among diverse chicken lines in response to Salmonella challenge

dc.contributor.author Kaiser, Michael
dc.contributor.author Hsieh, John
dc.contributor.author Kaiser, Pete
dc.contributor.author Lamont, Susan
dc.contributor.department Animal Science
dc.date.accessioned 2021-12-20T16:32:59Z
dc.date.available 2021-12-20T16:32:59Z
dc.date.issued 2021-11-19
dc.description.abstract Salmonella enterica serovar Enteritidis is a bacterial pathogen that contributes to poultry production losses and human foodborne illness. The bacterium elicits a broad immune response involving both the innate and adaptive components of the immune system. Coordination of the immune response is largely directed by cytokines. The objective of the current study was to characterize the expression of a select set of cytokines and regulatory immune genes in three genetically diverse chicken lines after infection with S. Enteritidis. Leghorn, Fayoumi and broiler day-old chicks were orally infected with pathogenic S. Enteritidis or culture medium. At 2 and 18 h postinfection, spleens and ceca were collected and mRNA expression levels for 7 genes (GM-CSF, IL2, IL15, TGF-β1, SOCS3, P20K, and MHC class IIβ) were evaluated by real-time quantitative PCR. Genetic line had a significant effect on mRNA expression levels of IL15, TGF-β1, SOCS3 and P20K in the spleen and on P20K and MHC class IIβ in the cecum. Comparing challenged vs. unchallenged birds, the expression of SOCS3 and P20K mRNA were significantly higher in the spleen and cecum, while MHC class IIβ mRNA was significantly lower in spleen. Combining the current RNA expression results with those of previously reported studies on the same samples reveals distinct RNA expression profiles among the three genetic chicken lines and the 2 tissues. This study illustrates that these diverse genetic lines have distinctively different immune response to S. Enteritidis challenge within the spleen and the cecum.
dc.description.comments This article is published as Kaiser, Michael G., John Hsieh, Pete Kaiser, and Susan J. Lamont. "Differential Immunological Response Detected in mRNA Expression Profiles Among Diverse Chicken Lines in Response to Salmonella Challenge." Poultry Science (2021): 101605. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/avVOD3dr
dc.language.iso en
dc.publisher © 2021 The Authors
dc.source.uri https://doi.org/10.1016/j.psj.2021.101605 *
dc.subject chicken
dc.subject Salmonella enteritidis
dc.subject immune response
dc.subject genetic difference
dc.subject.disciplines DegreeDisciplines::Life Sciences::Animal Sciences::Poultry or Avian Science
dc.subject.disciplines DegreeDisciplines::Life Sciences::Genetics and Genomics
dc.title Differential immunological response detected in mRNA expression profiles among diverse chicken lines in response to Salmonella challenge
dc.type Article
dspace.entity.type Publication
relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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