JIL-1 H3S10 Tandem Kinase's role in Embryogenesis
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2024-05
Authors
Butler, Jacob
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Johansen, Kristen
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JIL-1, a histone-3 serine-10 tandem kinase, is a protein needed to maintain chromosome structure and gene expression. It can be found in the decondensed regions of the chromosome. In order to study and characterize the protein, a JIL-1 mutation removing all function was created, also known as a null mutant. Our JIL-1 Z2 homozygous mutation does not express any JIL-1 protein and Drosophila melanogaster with the mutation do not live past the third instar stage of life. However, in a cross including the small duplication, DpE1,Y+, Z2 homozygous lethality was partially rescued, allowing larvae to grow past the third instar stage. Proceeding a series of mapping crosses, the region responsible for the rescue was narrowed down to a portion containing Achaete Scute-Complex. The Achaete Scute-Complex consists of four genes, achaete, scute, lethal of scute and asense, which contribute to neuroblast determination. Previous data suggest by increasing the expression of one or more genes in the Achaete Scute Complex, Z2 homozygous lethality can partially rescue allowing larvae to grow past the third instar stage.This suggests JIL-1 plays a role in neural development. Therefore, in the study I will explore the lack of JIL-1 and the effects on neural development in embryogenesis.
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