Genomic Regions and Candidate Genes Affecting Response to Heat Stress with Newcastle Virus Infection in Commercial Layer Chicks Using Chicken 600K Single Nucleotide Polymorphism Array

dc.contributor.author Wang, Ying
dc.contributor.author Saelao, Perot
dc.contributor.author Chanthavixay, Ganrea
dc.contributor.author Gallardo, Rodrigo A.
dc.contributor.author Wolc, Anna
dc.contributor.author Fulton, Janet E.
dc.contributor.author Dekkers, Jack M.
dc.contributor.author Lamont, Susan
dc.contributor.author Kelly, Terra R.
dc.contributor.author Zhou, Huaijun
dc.contributor.department Department of Animal Science
dc.date.accessioned 2024-03-04T14:09:00Z
dc.date.available 2024-03-04T14:09:00Z
dc.date.issued 2024-02-24
dc.description.abstract Heat stress results in significant economic losses to the poultry industry. Genetics plays an important role in chickens adapting to the warm environment. Physiological parameters such as hematochemical parameters change in response to heat stress in chickens. To explore the genetics of heat stress resilience in chickens, a genome-wide association study (GWAS) was conducted using Hy-Line Brown layer chicks subjected to either high ambient temperature or combined high temperature and Newcastle disease virus infection. Hematochemical parameters were measured during three treatment phases: acute heat stress, chronic heat stress, and chronic heat stress combined with NDV infection. Significant changes in blood parameters were recorded for 11 parameters (sodium (Na+, potassium (K+), ionized calcium (iCa2+), glucose (Glu), pH, carbon dioxide partial pressure (PCO2), oxygen partial pressure (PO2), total carbon dioxide (TCO2), bicarbonate (HCO3), base excess (BE), and oxygen saturation (sO2)) across the three treatments. The GWAS revealed 39 significant SNPs (p < 0.05) for seven parameters, located on Gallus gallus chromosomes (GGA) 1, 3, 4, 6, 11, and 12. The significant genomic regions were further investigated to examine if the genes within the regions were associated with the corresponding traits under heat stress. A candidate gene list including genes in the identified genomic regions that were also differentially expressed in chicken tissues under heat stress was generated. Understanding the correlation between genetic variants and resilience to heat stress is an important step towards improving heat tolerance in poultry.
dc.description.comments This article is published as Wang, Ying, Perot Saelao, Ganrea Chanthavixay, Rodrigo A. Gallardo, Anna Wolc, Janet E. Fulton, Jack M. Dekkers, Susan J. Lamont, Terra R. Kelly, and Huaijun Zhou. 2024. "Genomic Regions and Candidate Genes Affecting Response to Heat Stress with Newcastle Virus Infection in Commercial Layer Chicks Using Chicken 600K Single Nucleotide Polymorphism Array" International Journal of Molecular Sciences 25, no. 5: 2640. https://doi.org/10.3390/ijms25052640. Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/jrl8KROr
dc.language.iso en
dc.publisher Multidisciplinary Digital Publishing Institute
dc.source.uri https://doi.org/10.3390/ijms25052640 *
dc.subject.disciplines DegreeDisciplines::Life Sciences::Animal Sciences::Poultry or Avian Science
dc.subject.disciplines DegreeDisciplines::Life Sciences::Genetics and Genomics
dc.subject.keywords heat stress
dc.subject.keywords GWAS
dc.subject.keywords NDV
dc.subject.keywords chicken
dc.subject.keywords QTL
dc.title Genomic Regions and Candidate Genes Affecting Response to Heat Stress with Newcastle Virus Infection in Commercial Layer Chicks Using Chicken 600K Single Nucleotide Polymorphism Array
dc.type article
dspace.entity.type Publication
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relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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