Canine Peripheral Blood Lymphocyte Phenotyping by 7-Color Multiparameter Flow Cytometry

dc.contributor.author Platt, Ratree
dc.contributor.author Ng, Terry
dc.contributor.author Roth, James
dc.contributor.author Glover, Sherry
dc.contributor.author Roof, Michael
dc.contributor.author Kimura, Kayoko
dc.contributor.author Roth, James
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-17T05:03:16.000
dc.date.accessioned 2020-07-07T05:15:32Z
dc.date.available 2020-07-07T05:15:32Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2013
dc.date.issued 2013-08-01
dc.description.abstract <p><strong><strong>Objective:</strong></strong> To characterize baseline canine lymphocyte phenotypes including lymphocytes coexpressing multiple markers by novel 7-color multiparameter flow cytometry. <strong> <strong>Study Design:</strong></strong> Fresh canine peripheral blood lymphocytes of 79 healthy 26-week-old Beagle or Beagle-mix dogs were stained and analyzed. <strong> <strong>Results:</strong></strong> The high number of samples and acquired flow data (averaging 1.9x105 cells/sample) allowed the detection of minor lymphocyte subsets coexpressing multiple lymphocyte markers. The averaged percentages of major lymphocyte subsets of CD3+, CD4+, CD8+, CD21+ and gd TCR+ cells from this study were 74.0, 43.6, 14.3, 9.6, and 0.2, respectively, which were comparable but uniquely different from other reports as they were simultaneously detected in the same sample. We demonstrated that the commonly used CD21 and CD3 monoclonal antibody (mAb) clones, previously recommended not to be used in the same staining, could and should be used together with the proper steps of lymphocyte gating. We found a high percentage (10.3%) of unidentified CD21–CD3+CD4–CD8–gdTCR– lymphocyte subset that has never been reported. The intensive gating strategy and the mean percentages of each lymphocyte subset to their parent subsets and to the total lymphocyte population are presented and discussed. <strong> <strong>Conclusion:</strong></strong> The canine lymphocyte phenotypes were fully characterized. This novel multiparameter flow cytometry method is a powerful approach to in-crease the accuracy of lymphocyte phenotyping in dogs.</p>
dc.description.comments <p>This article is from <em>Analytical and Quantitative Cytopathology and Histopathology</em> 35 (2013): 197. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/92/
dc.identifier.articleid 1091
dc.identifier.contextkey 7807255
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/92
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92402
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/92/2013_Roth_CaninePeripheral.pdf|||Sat Jan 15 02:29:41 UTC 2022
dc.subject.disciplines Small or Companion Animal Medicine
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords Beagle dog
dc.subject.keywords canine diseases
dc.subject.keywords canine lymphocytes
dc.subject.keywords flow cytometry
dc.subject.keywords lymphocyte phenotyping
dc.title Canine Peripheral Blood Lymphocyte Phenotyping by 7-Color Multiparameter Flow Cytometry
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 909dd0b2-ec0a-41e2-b4e0-9e5ff76b7622
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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