papA Gene of Avian Pathogenic Escherichia coli

dc.contributor.author Kariyawasam, Subhashinie
dc.contributor.author Nolan, Lisa
dc.contributor.author Nolan, Lisa
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-13T10:45:59.000
dc.date.accessioned 2020-07-07T05:15:13Z
dc.date.available 2020-07-07T05:15:13Z
dc.date.copyright Sat Jan 01 00:00:00 UTC 2011
dc.date.embargo 2013-05-08
dc.date.issued 2011-12-01
dc.description.abstract <p>P fimbrial adhesins may be associated with the virulence of avian pathogenic <em>Escherichia coli</em> (APEC). However, most APECs are unable to express P fimbriae even when they are grown under conditions that favor P fimbrial expression. This failure can be explained by the complete absence of the <em>pap</em> operon or the presence of an incomplete <em>pap</em> operon in Pap-negative APEC strains. In the present study, we analyzed the <em>pap</em> operon, specifically the<em>papA</em> gene that encodes the major fimbrial shaft, to better understand the <em>pap</em> gene cluster at the genetic level. First, by PCR, we examined a collection of 500 APEC strains for the presence of 11 genes comprising the <em>pap</em> operon. Except for <em>papA</em>, all the other genes of the operon were present in 38% to 41.2% of APEC, whereas the <em>papA</em> was present only in 10.4% of the APEC tested. Using multiplex PCR to probe for allelic variants of <em>papA</em>, we sought to determine if the low prevalence of <em>papA</em> among APEC was related to genetic heterogeneity of the gene itself. It was determined that the <em>papA</em> of APEC always belongs to the F11 allelic variant. Finally, we sequenced the ‘<em>papA</em> region’ from two <em>papA</em>-negative strains, both of which contain all the other genes of the <em>pap</em> operon. Interestingly, both strains had an 11,104-bp contig interrupting <em>papA</em> at the 281-bp position. This contig harbored a streptomycin resistance gene and a classic Tn<em>10</em> transposon containing the genes that confer tetracycline resistance. However, we noted that the <em>papA</em> gene of every <em>papA</em>-negative APEC strain was not interrupted by an 11,104-bp contig. It is likely that transposons bearing antibiotic resistance genes have inserted within <em>pap</em> gene cluster of some APEC strains, and such genetic events may have been selected for by antibiotic use.</p>
dc.description.comments <p>This article is from <em>Avian Diseases</em> 55, no. 4 (2011): 532–538, doi:<a href="http://dx.doi.org.proxy.lib.iastate.edu/10.1637/9663-011911-Reg.1" target="_blank">10.1637/9663-011911-Reg.1</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/46/
dc.identifier.articleid 1039
dc.identifier.contextkey 4117359
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/46
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92351
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/46/2011_KariyawasamS_papAGeneAvianPathogenic.pdf|||Sat Jan 15 00:21:59 UTC 2022
dc.source.uri 10.1637/9663-011911-Reg.1
dc.subject.disciplines Genomics
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords adherence
dc.subject.keywords avian pathogenic Escherichia coli
dc.subject.keywords P fimbriae
dc.subject.keywords papA
dc.subject.keywords pap operon
dc.subject.keywords transposon
dc.subject.keywords virulence
dc.title papA Gene of Avian Pathogenic Escherichia coli
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 9e7506b4-e945-47cf-9195-e814dac6c9fd
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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