Calsensin: a novel calcium-binding protein expressed in a subset of peripheral leech neurons fasciculating in a single axon tract

dc.contributor.author Briggs, Kristen
dc.contributor.author Johansen, Kristen
dc.contributor.author Silvers, Andrea
dc.contributor.author Johansen, Kristen
dc.contributor.author Johansen, Jorgen
dc.contributor.department Zoology and Genetics
dc.date 2019-07-09T02:31:52.000
dc.date.accessioned 2020-07-07T05:17:01Z
dc.date.available 2020-07-07T05:17:01Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 1995
dc.date.issued 1995-06-01
dc.description.abstract <p>The mAb lan3-6 recognizes a cytosolic antigen which is selectively expressed in the growth cones and axons of a small subset of peripheral sensory neurons fasciculating in a single tract common to all hirudinid leeches. We have used this antibody to clone a novel EF-hand calcium-binding protein, calsensin, by screening an expression vector library. A full-length clone of 1.1 kb identified by the antibody was isolated and sequenced. In situ hybridizations with calsensin probes and antibody staining using new polyclonal antisera generated against calsensin sequence demonstrate that calsensin indeed corresponds to the lan3-6 antigen. Calsensin consists of 83 residues with a calculated molecular mass of 9.1 kD that contains two helix-loop-helix domains. The calcium-binding domains are likely to be functional in vivo since a fusion protein derived from the calsensin clone binds 45Ca2+ in vitro. Immunoaffinity purification experiments with the lan3-6 antibody shows that a large 200,000 M(r) protein selectively copurifies with calsensin in two different leech species. These results suggest that calsensin may be functioning as a trigger protein which interacts with the larger protein. These data are consistent with the hypothesis that calsensin may mediate calcium-dependent signal transduction events in the growth cones and axons of this small group of sensory neurons which fasciculate in a single axon tract.</p>
dc.description.comments <p>This article is published as Briggs, Kristen Kay, Andrea J. Silvers, Kristen M. Johansen, and Jørgen Johansen. "Calsensin: a novel calcium-binding protein expressed in a subset of peripheral leech neurons fasciculating in a single axon tract." <em>The Journal of cell biology </em>129, no. 5 (1995): 1355-1362. doi: <a href="http://dx.doi.org/10.1083/jcb.129.5.1355" target="_blank">10.1083/jcb.129.5.1355</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/zool_pubs/54/
dc.identifier.articleid 1048
dc.identifier.contextkey 14512259
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath zool_pubs/54
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92666
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/zool_pubs/54/1995_Johansen_CalsensinNovel.pdf|||Sat Jan 15 00:53:23 UTC 2022
dc.source.uri 10.1083/jcb.129.5.1355
dc.subject.disciplines Biochemistry, Biophysics, and Structural Biology
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Genetics
dc.title Calsensin: a novel calcium-binding protein expressed in a subset of peripheral leech neurons fasciculating in a single axon tract
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 3829d759-f8ec-4502-a63a-1655d3b70ef5
relation.isOrgUnitOfPublication 4a2929da-5374-4338-b62f-f5fd9e156ef9
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