Probiotic Potential and Antimicrobial Activity of Enterococcus faecium Isolated from Chicken Caecal and Fecal Samples Abu Hafsa, Salma Brehm-Stecher, Byron Mendonca, Aubrey Brehm-Stecher, Byron Hassan, A. A. Ibrahim, S. A.
dc.contributor.department Food Science and Human Nutrition 2018-02-19T07:32:46.000 2020-06-30T03:58:55Z 2020-06-30T03:58:55Z Thu Jan 01 00:00:00 UTC 2015 2015-04-01
dc.description.abstract <p>Enterococci are important inhabitants of the animal intestine and are widely used in probiotic products. A probiotic strain is expected to possess several desirable properties in order to exert beneficial effects. Therefore, the objective of this study was to isolate, characterize and identify Enterococcus sp. from chicken cecal and fecal samples to determine potential probiotic properties. Enterococci were isolated from chicken ceca and feces of thirty three clinically healthy chickens from a local farm. In vitro studies were performed to assess antibacterial activity of the isolated LAB (using agar well diffusion and cell free supernatant broth technique against Salmonella enterica serotype Enteritidis), survival in acidic conditions, resistance to bile salts, and their survival during simulated gastric juice conditions at pH 2.5. Isolates were identified by biochemical carbohydrate fermentation patterns using an API 50 CHL kit and API ZYM kits and by sequenced 16S rDNA. An isolate belonging to E. faecium species exhibited inhibitory effect against S. enteritidis. This isolate producing a clear zone as large as 10.30 mm or greater and was able to grow in the coculture medium and at the same time, inhibited the growth S. enteritidis. In addition, E. faecium exhibited significant resistance under highly acidic conditions at pH 2.5 for 8 h and survived well in bile salt at 0.2% for 24 h and showing ability to survive in the presence of simulated gastric juice at pH 2.5. Based on these results, E. faecium isolate fulfills some of the criteria to be considered as a probiotic strain and therefore, could be used as a feed additive with good potential for controlling S. Enteritidis in chickens. However, in vivo studies are needed to determine the safety of the strain.</p>
dc.description.comments <p>This article is published as 5. Abu Hafsa, S.H., Mendonca, A., Brehm-Stecher, B., Hassan, A.A. and S.A. Ibrahim. Probiotic potential and antimicrobial activity of Enterococcus faecium isolated from chicken caecal and fecal samples. International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering 9(4): 350-54 (2015). Posted with permission.</p>
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dc.identifier archive/
dc.identifier.articleid 1174
dc.identifier.contextkey 11375695
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath fshn_ag_pubs/173
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 21:20:09 UTC 2022
dc.subject.disciplines Food Science
dc.subject.disciplines Human and Clinical Nutrition
dc.subject.disciplines Immunology of Infectious Disease
dc.subject.disciplines Molecular, Genetic, and Biochemical Nutrition
dc.subject.disciplines Organismal Biological Physiology
dc.subject.disciplines Plant Sciences
dc.subject.keywords Acid tolerance
dc.subject.keywords antimicrobial activity
dc.subject.keywords Enterococcus faecium
dc.subject.keywords probiotic
dc.title Probiotic Potential and Antimicrobial Activity of Enterococcus faecium Isolated from Chicken Caecal and Fecal Samples
dc.type article
dc.type.genre article
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