Why do Ladybugs Smell Bad? In‐vivo Quantification of Odorous Insect Kairomones with SPME and Multidimensional GC‐MS‐Olfactometry

dc.contributor.author Koziel, Jacek
dc.contributor.author Cai, Lingshuang
dc.contributor.author Koziel, Jacek
dc.contributor.author O'Neal, Matthew
dc.contributor.author O'Neal, Matthew
dc.contributor.department Agricultural and Biosystems Engineering
dc.date 2018-02-13T06:11:43.000
dc.date.accessioned 2020-06-29T22:32:37Z
dc.date.available 2020-06-29T22:32:37Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 2009
dc.date.embargo 2013-02-13
dc.date.issued 2009-04-01
dc.description.abstract <p>Winemakers, small fruit growers, and homeowners are concerned with noxious compounds released by multicolored Asian ladybird beetles (<em>Harmonia axyridis</em>, Coleoptera: Coccinellidae). New method based on headspace solid phase microextraction (HS‐SPME) coupled with multidimensional gas chromatography mass spectrometry—olfactometry (MDGC‐MS‐O) system was developed for extraction, isolation and simultaneous identification of compounds responsible for the characteristic odor of live <em>H. axyridis</em>. Four methoxypyrazines (MPs) were identified in headspace volatiles of live<em>H. axyridis</em> as those responsible for the characteristic odor: 2, 5‐dimethy1‐3‐methoxypyrazine (DMMP), 2‐isopropy1‐3‐methoxypyrazine (IPMP), 2‐sec‐buty1‐3‐methoxypyrazine (SBMP), and 2‐isobuty1‐3‐methoxypyrazine (IBMP). To the best of our knowledge this is the first report of <em>H. axyridis</em> releasing DMMP and the first report of this compound being a component of the <em>H. axyridis</em> characteristic odor. Quantification of three MPs (IPMP, SBMP and IBMP) emitted from live <em>H. axyridis</em> were performed using external calibration with HS‐SPME and direct injections. A linear relationship (R<sup>2</sup>>0.9958for all 3 MPs) between MS response and concentration of standard was observed over a concentration range from 0.1 ng L<sup>−1</sup> to 0.05 <em>μ</em>g L<sup>−1</sup> for HS‐SPME‐GC‐MS. The method detection limits (MDL) based on multidimensional GC‐MS approach for three MPs were estimated to be between 0.020 ng L<sup>−1</sup>. to 0.022 ng L<sup>−1</sup>. This methodology is applicable for<em>in vivo</em> determination of odor‐causing chemicals associated with emissions of volatiles from insects.</p>
dc.description.comments <p>Copyright 2009 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics.</p> <p>This article appeared in <em>AIP Conference Proceedings </em>1137 (2009): 245–248 and may be found at <a href="http://link.aip.org/link/doi/10.1063/1.3156517">http://dx.doi.org/10.1063/1.3156517</a>.</p>
dc.identifier archive/lib.dr.iastate.edu/abe_eng_conf/229/
dc.identifier.articleid 1231
dc.identifier.contextkey 3694728
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath abe_eng_conf/229
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/238
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/abe_eng_conf/229/Koziel_2009_WhyLadybugsSmell.pdf|||Fri Jan 14 22:44:48 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Bioresource and Agricultural Engineering
dc.subject.keywords chemical sensors
dc.subject.keywords electrochemical sensors
dc.subject.keywords mass spectroscopy
dc.title Why do Ladybugs Smell Bad? In‐vivo Quantification of Odorous Insect Kairomones with SPME and Multidimensional GC‐MS‐Olfactometry
dc.type article
dc.type.genre conference
dspace.entity.type Publication
relation.isAuthorOfPublication 76fc5589-51f8-4f3c-885c-e25d8037d641
relation.isAuthorOfPublication cdca6b0a-65c4-45dc-a6e4-4f0f1035f453
relation.isOrgUnitOfPublication 8eb24241-0d92-4baf-ae75-08f716d30801
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