Antimicrobial agents are being used in modern swine production worldwide, generating concern in regards to the development of antimicrobial resistance. Identifying efficient alternatives has therefore become a subject of interest. The aim of this study was to explore the impact of routinely used vaccination as an alternative to antimicrobial consumption in weaner pig herds. The hypothesis was that herds with increased use of vaccination would have a lower antimicrobial consumption. Data were obtained from the Danish VetStat database in which prescriptions of medication for livestock are recorded as well as the Danish Central Husbandry Register. All Danish one-site pig herds, active in year 2013, with >50 sows and >200 weaners were selected for the study. Initially, data were analysed using a univariable model, and secondly a multivariable linear regression model was applied. The analyses included use of three different vaccines against Porcine Circovirus Type 2 (PCV2), Mycoplasma hyopneumoniae (M_HYO) and Lawsonia intracellularis (LAW), respectively, as well as annual production measured as number of weaners produced in a year. The outcome was the average antimicrobial consumption measured in animal daily does (ADD) per weaner pig. Out of the 1,513 herds selected for the study, 1,415 herds had antimicrobials prescribed for gastrointestinal disorders, and 836 for respiratory disorders. PCV2 vaccine was used in 880 herds, M_HYO vaccine in 787 and LAW vaccine was the least used, with 115 herds using it. The results suggested that antimicrobials to some extent were being used for other disease categories than those officially prescribed by the veterinarians. On average, herds using the different combinations of vaccines had higher use of antimicrobials than herds not using the vaccines – probably as a result of health problems in the herds existing prior to the initiation of vaccine use. Information about vaccination protocols, health status, biosecurity, and management practices was not available limiting the ability to assess causality.