Transcriptomic and anatomical complexity of primary, seminal, and crown roots highlight root type-specific functional diversity in maize (Zea mays L.)

Date
2016-01-01
Authors
Tai, Huanhuan
Lu, Xin
Nettleton, Dan
Opitz, Nin
Marcon, Caroline
Paschold, Anja
Lithio, Andrew
Nettleton, Dan
Hochholdinger, Frank
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Research Projects
Organizational Units
Statistics
Organizational Unit
Journal Issue
Series
Department
Statistics
Abstract

Maize develops a complex root system composed of embryonic and post-embryonic roots. Spatio-temporal differences in the formation of these root types imply specific functions during maize development. A comparative transcriptomic study of embryonic primary and seminal, and post-embryonic crown roots of the maize inbred line B73 by RNA sequencing along with anatomical studies were conducted early in development. Seminal roots displayed unique anatomical features, whereas the organization of primary and crown roots was similar. For instance, seminal roots displayed fewer cortical cell files and their stele contained more meta-xylem vessels. Global expression profiling revealed diverse patterns of gene activity across all root types and highlighted the unique transcriptome of seminal roots. While functions in cell remodeling and cell wall formation were prominent in primary and crown roots, stress-related genes and transcriptional regulators were over-represented in seminal roots, suggesting functional specialization of the different root types. Dynamic expression of lignin biosynthesis genes and histochemical staining suggested diversification of cell wall lignification among the three root types. Our findings highlight a cost-efficient anatomical structure and a unique expression profile of seminal roots of the maize inbred line B73 different from primary and crown roots.

Comments

This article is published as Tai, Huanhuan, Xin Lu, Nina Opitz, Caroline Marcon, Anja Paschold, Andrew Lithio, Dan Nettleton, and Frank Hochholdinger. "Transcriptomic and anatomical complexity of primary, seminal, and crown roots highlight root type-specific functional diversity in maize (Zea mays L.)." Journal of Experimental Botany 67, no. 4 (2015): 1123-1135. doi: 10.1093/jxb/erv513.

Description
Keywords
Citation
DOI
Collections