First Trimester Prediction of Preterm Birth in Patient Plasma with Machine-Learning-Guided Raman Spectroscopy and Metabolomics
Date
2023-08-07
Authors
Synan, Lilly
Ghazvini, Saman
Uthaman, Saji
Cutshawa, Gabriel
Lee, Che-Yu
Waite, Joshua
Wen, Xiaona
Lin, Eugene
Santillan, Mark
Santillan, Donna
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American Chemical Society
Abstract
Preterm birth (PTB) is the leading cause of infant deaths globally. Current clinical measures often fail to identify women who may deliver preterm. Therefore, accurate screening tools are imperative for early prediction of PTB. Here, we show that Raman spectroscopy is a promising tool for studying biological interfaces, and we examine differences in the maternal metabolome of the first trimester plasma of PTB patients and those that delivered at term (healthy). We identified fifteen statistically significant metabolites that are predictive of the onset of PTB. Mass spectrometry metabolomics validates the Raman findings identifying key metabolic pathways that are enriched in PTB. We also show that patient clinical information alone and protein quantification of standard inflammatory cytokines both fail to identify PTB patients. We show for the first time that synergistic integration of Raman and clinical data guided with machine learning results in an unprecedented 85.1% accuracy of risk stratification of PTB in the first trimester that is currently not possible clinically. Correlations between metabolites and clinical features highlight the body mass index and maternal age as contributors of metabolic rewiring. Our findings show that Raman spectral screening may complement current prenatal care for early prediction of PTB, and our approach can be translated to other patient-specific biological interfaces.
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This is a manuscript of an article published as Synan, Lilly, Saman Ghazvini, Saji Uthaman, Gabriel Cutshaw, Che-Yu Lee, Joshua Waite, Xiaona Wen et al. "First Trimester Prediction of Preterm Birth in Patient Plasma with Machine-Learning-Guided Raman Spectroscopy and Metabolomics." ACS Applied Materials & Interfaces 15, no. 32 (2023): 38185-38200. doi: https://doi.org/10.1021/acsami.3c04260. Posted with Permission. Copyright © 2023 American Chemical Society.