Comparison of enrichment schemes for the isolation of Yersinia enterocolitica
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Abstract
The food-borne pathogen Yersinia enterocolitica (YE) has been repeatedly linked to swine and is a world wide food safety risk. Microbiological culture methods for YE lack some functionality as the current gold standard requires a 21 day cold enrichment in phosphate buffered saline (PBS). In this study a shortened enrichment scheme using a higher incubation temperature and a more selective media (LB-BSI) was compared to PBS for the isolation of YE from swine feces. Both enrichments produced similar overall isolation rates but had a low level of agreement (kappa=0.11) for determining an individual pig’s YE status. LB-BSI yielded a sensitivity and specificity of 22.7% and 87.2% respectively when compared to PBS. At the time of submission, further genotyping and serotyping assays are pending that may aid in discerning the source of disagreement between the methods.