The objective of this research is to examine, for the first time, the hot splash protection provided by the commercially available children’s sleepwear. Three types of commercially available children’s sleepwear, knitted in different fabric structures with different fiber composition, were evaluated. Polyester fabrics, due to better wicking property, spread hot water faster in both horizontal and vertical directions. As a result, a larger body surface area of the wearer would get burnt quickly. Cotton/modacrylic interlock fabrics, due to cotton excellent absorption of water and high thickness of the interlock structure, less body surface area was affected, indicating superior splash protection provided. The performance could be further improved if the fabric were made modacrylic rich. This research would provide a critical insight into the selection of fiber and fabric structure in designing this sophisticated class of fabric for superior flame and splash protection to children.