Identification of transactivation-response sequences in the long terminal repeat of bovine immunodeficiency-like virus
Transient expression assays using the reporter gene that encodes chloramphenicol acetyltransferase were used to identify cis-acting sequences necessary for bovine immunodeficiency-like virus (BIV) transactivation. Computer analyses identified two RNA stem-loop structures located immediately downstream of the transcription start site in the long terminal repeat. Deletion analysis of the long terminal repeat indicated that sequences containing the proximal stem-loop structure located between +4 and +31 are required for virus-specific transactivation. Therefore, BIV likely utilizes a mechanism of transactivation similar to that of the human and simian lentiviruses.
This article is published as Carpenter, S., S. A. Nadin-Davis, Y. Wannemuehler, and J. A. Roth. "Identification of transactivation-response sequences in the long terminal repeat of bovine immunodeficiency-like virus." Journal of virology 67, no. 7 (1993): 4399-4403. Posted with permission.