Effects of the synthetic selectin inhibitor TBC1269 on tissue damage during acute Mannheimia haemolytica-induced pneumonia in neonatal calves

Abstract

<p><strong>Objective</strong>—To determine effects of the selectin inhibitor TBC1269 on neutrophil-mediated pulmonary damage during acute <em>Mannheimia haemolytica-induced</em>pneumonia in newborn calves.</p> <p><strong>Animals</strong>—Eighteen 1- to 3-day-old colostrumdeprived calves.</p> <p><strong>Procedure</strong>—<em>Mannheimia haemolytica</em> or saline (0.9% NaCl) solution was inoculated in both cranial lung lobes of 12 and 6 calves, respectively. Calves were euthanatized 2 (saline, n = 3; <em>M haemolytica</em>, n = 4) or 6 hours (saline, n = 3; <em>M haemolytica</em>, n = 8) after inoculation. Four <em>M haemolytica</em>-inoculated calves euthanatized at 6 hours also received TBC1269 (25 mg/kg, IV) 30 minutes before and 2 hours after inoculation. Conjugated diene (CD) concentrations, inducible nitric oxide synthase (iNOS) expression, and apoptotic cell counts were determined in lung specimens collected during necropsy.</p> <p><strong>Results</strong>—Conjugated diene concentrations were significantly increased in all <em>M haemolytica</em>-inoculated groups, compared with saline-inoculated groups. Calves treated with TBC1269 had decreased concentrations of CD, compared with untreated calves, although the difference was not significant. Number of apoptotic neutrophils and macrophages increased significantly in TBC1269-treated calves, compared with untreated calves. Inducible nitric oxide synthase was expressed by epithelial cells and leukocytes. However, iNOS was less abundant in airway epithelial cells associated with inflammatory exudates. Degree of iNOS expression was similar between TBC1269-treated and untreated calves.</p> <p><strong>Conclusions</strong>—<em>Mannheimia haemolytica</em> infection in neonatal calves resulted in pulmonary tissue damage and decreased epithelial cell iNOS expression. The selectin inhibitor TCB1269 altered, but did not completely inhibit, neutrophil-mediated pulmonary damage.</p>