Depletion of alveolar glycogen corresponds with immunohistochemical development of CD208 antigen expression in perinatal lamb lung

dc.contributor.author Ackermann, Mark
dc.contributor.author Meyerholz, David
dc.contributor.author DeGraaf, James
dc.contributor.author Gallup, Jack
dc.contributor.author Gallup, Jack
dc.contributor.author Olivier, Alicia
dc.contributor.author Ackermann, Mark
dc.contributor.department Veterinary Pathology
dc.date 2018-02-13T06:22:08.000
dc.date.accessioned 2020-07-07T05:15:51Z
dc.date.available 2020-07-07T05:15:51Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2006
dc.date.embargo 2013-02-26
dc.date.issued 2006-11-01
dc.description.abstract <p>CD208 DC lysosomal-associated protein is a marker of activated human dendritic cells; however, recently it was described as a marker of adult type II pneumocytes in many species including humans and sheep. Our hypothesis was that CD208 is developmentally regulated in lung pneumocytes. Lamb lungs at varying stages of development were stained immunohistochemically for CD208 and with Nile red (a fluorescent stain for lamellar bodies of type II cells) along with pulmonary markers of maturation (glycogen stores and surfactant protein A [SP-A] expression) or proliferation (Ki-67). CD208 staining and Nile red were localized to rare pneumocytes in young fetal lambs (day 115), increasing in frequency and stain intensity with age. Periodic acid-Schiff staining of glycogen granules was most prominent in the young lambs (day 115) with reduced staining through advancing lung development. SP-A was detected in pulmonary epithelia and staining in alveoli increased through gestation with decreased staining at 2 weeks of age. Intranuclear Ki-67 staining decreased through late gestation but was increased in 2-week-old lambs. Ontogeny of CD208 staining and depletion of glycogen were correlated (<em>p</em><0.0001) and consistent with the premise that CD208 is localized to developing lamellar bodies. The findings suggest that CD208 antigen expression may serve as a marker for pneumocyte maturation in the developing fetal lung.</p>
dc.description.comments <p>The final, definitive versin of this paper has been published in <em>Journal of Histochemistry and</em> Cytochemistry,<em> </em>54/11, 11/2006 by SAGE Publications Ltd, All rights reserved, © The Histochemical Society, Inc.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vpath_pubs/33/
dc.identifier.articleid 1027
dc.identifier.contextkey 3788055
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vpath_pubs/33
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92457
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vpath_pubs/33/2006_Meyerholz_DepletionAlveolarGlycogen.pdf|||Fri Jan 14 23:37:19 UTC 2022
dc.source.uri 10.1369/jhc.6A7002.2006
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.keywords CD208
dc.subject.keywords DC-lysosomal-associated
dc.subject.keywords protein
dc.subject.keywords lamb
dc.subject.keywords lamellar body
dc.subject.keywords ontogeny
dc.subject.keywords type II pneumocyte
dc.title Depletion of alveolar glycogen corresponds with immunohistochemical development of CD208 antigen expression in perinatal lamb lung
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f
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