Performance of a Differentiation of Infected from Vaccinated Animals (DIVA) Classical Swine Fever Virus (CSFV) Serum and Oral Fluid Erns Antibody AlphaLISA Assay

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2023-12-09
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Panyasing, Yaowalak
Thanawongnuwech, Roongroje
Prakobsuk, Phakawan
Kawilaphan, Yanee
Kittawornrat, Apisit
Cheng, Ting-Yu
Zimmerman, Jeffrey
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Multidisciplinary Digital Publishing Institute
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Giménez-Lirola, Luis
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Veterinary Diagnostic and Production Animal Medicine
Abstract
Classical swine fever virus (CSFV) is an OIE-listed disease that requires effective surveillance tools for its detection and control. The aim of this study was to develop and evaluate the diagnostic performance of a novel CSFV Erns IgG AlphaLISA for both serum and oral fluid specimens that would likewise be compatible with the use of CSFV E2 DIVA vaccines. Test performance was evaluated using a panel of well-characterized serum (n = 760) and individual (n = 528) or pen-based (n = 30) oral fluid samples from four groups of animals: (1) negative controls (n = 60 pigs); (2) inoculated with ALD strain wild-type CSFV (n = 30 pigs); (3) vaccinated with LOM strain live CSFV vaccine (n = 30 pigs); and (4) vaccinated with live CSFV marker vaccine on commercial farms (n = 120 pigs). At a cutoff of S/P ≥ 0.7, the aggregate estimated diagnostic sensitivities and specificities of the assay were, respectively, 97.4% (95% CI 95.9%, 98.3%) and 100% for serum and 95.4% (95% CI 92.9%, 97.0%) and 100% for oral fluid. The Erns IgG antibody AlphaLISA combined DIVA capability with solid diagnostic performance, rapid turnaround, ease of use, and compatibility with both serum and oral fluid specimens.
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This article is published as Panyasing, Yaowalak, Luis Gimenez-Lirola, Roongroje Thanawongnuwech, Phakawan Prakobsuk, Yanee Kawilaphan, Apisit Kittawornrat, Ting-Yu Cheng, and Jeffrey Zimmerman. "Performance of a Differentiation of Infected from Vaccinated Animals (DIVA) Classical Swine Fever Virus (CSFV) Serum and Oral Fluid Erns Antibody AlphaLISA Assay." Animals 13, no. 24 (2023): 3802. doi: https://doi.org/10.3390/ani13243802. Copyright: © 2023 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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