Evaluation of a combinatorial approach to prion inactivation using an oxidizing agent, SDS, and proteinase K

dc.contributor.author Smith, Jodi
dc.contributor.author Nicholson, Eric
dc.contributor.author Greenlee, Justin
dc.contributor.department Veterinary Pathology
dc.date 2018-02-18T22:26:15.000
dc.date.accessioned 2020-07-07T05:16:11Z
dc.date.available 2020-07-07T05:16:11Z
dc.date.issued 2013-07-25
dc.description.abstract <p><h3>Background</h3></p> <p>Prions demonstrate an unusual resistance to methods effective at inactivating conventional microorganisms. This has resulted in a very tangible and difficult infection control challenge to the medical and veterinary communities, as well as animal agriculture and related industries. Currently accepted practices of harsh chemical treatments such as prolonged exposure to sodium hydroxide or sodium hypochlorite, or autoclaving are not suitable in many situations. Less caustic and more readily applicable treatments to contaminated environments are therefore desirable. We recently demonstrated that exposure of the RML scrapie agent to a commercial product containing sodium percarbonate (SPC-P) with or without sodium dodecyl sulfate (SDS) rendered PrP<sup>Sc</sup> sensitive to proteinase K (PK), but did not eliminate infectivity. The current study was designed to evaluate the efficacy of a combinatorial approach to inactivating prions by exposing RML-positive brain homogenate to SPC-P and SDS followed by PK. Treated samples were evaluated for PrP<sup>Sc</sup>-immunoreactivity by western blot, and residual infectivity by mouse bioassay. <h3>Results</h3></p> <p>Treatment of infected brain homogenate with SPC-P and SDS followed by PK exposure resulted in a 4–5 log<sub>10</sub> reduction in infectivity when bioassayed in <em>tg</em>a<em>20</em> mice. <h3>Conclusions</h3></p> <p>This study demonstrates that exposure of the RML scrapie agent to SPC-P and SDS followed by PK markedly reduces, but does not eliminate infectivity. The results of this study encourage further investigation into whether consecutive or concomitant exposure to sodium percarbonate, SDS, and a protease may serve as a viable and non-caustic option for prion inactivation.</p>
dc.description.comments <p>This article is published as Smith, Jodi D., Eric M. Nicholson, and Justin J. Greenlee. "Evaluation of a combinatorial approach to prion inactivation using an oxidizing agent, SDS, and proteinase K." BMC veterinary research 9, no. 1 (2013): 151. doi: <a href="https://doi.org/10.1186/1746-6148-9-151" target="_blank">10.1186/1746-6148-9-151</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vpath_pubs/86/
dc.identifier.articleid 1089
dc.identifier.contextkey 10717118
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vpath_pubs/86
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92515
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vpath_pubs/86/2013_Smith_EvalutaionCombinatorial.pdf|||Sat Jan 15 02:14:08 UTC 2022
dc.source.uri 10.1186/1746-6148-9-151
dc.subject.disciplines Veterinary Infectious Diseases
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.disciplines Veterinary Toxicology and Pharmacology
dc.subject.keywords Inactivation
dc.subject.keywords Prion
dc.subject.keywords Proteinase
dc.subject.keywords Scrapie
dc.subject.keywords Sodium dodecyl sulfate
dc.subject.keywords Sodium percarbonate
dc.title Evaluation of a combinatorial approach to prion inactivation using an oxidizing agent, SDS, and proteinase K
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 3a167321-8198-47f6-b147-25a983d1c364
relation.isOrgUnitOfPublication cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f
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