Dry-Milling and Fractionation of Transgenic Maize Seed Tissues with Green Fluorescent Protein as a Tissue Marker

dc.contributor.author Shepherd, C. T.
dc.contributor.author Vignaux, N.
dc.contributor.author Peterson, J. M.
dc.contributor.author Scott, Marvin
dc.contributor.author Scott, M. P.
dc.contributor.author Johnson, L. A.
dc.contributor.department Agronomy
dc.contributor.department Center for Crops Utilization Research
dc.contributor.department Genetics
dc.date 2018-02-18T14:50:44.000
dc.date.accessioned 2020-06-29T23:02:53Z
dc.date.available 2020-06-29T23:02:53Z
dc.date.issued 2008-01-01
dc.description.abstract <p>The efficiency of fractionating cereal grains (e.g., dry corn milling) can be evaluated and monitored by quantifying the proportions of seed tissues in each of the recovered fractions. The quantities of individual tissues are typically estimated using indirect methods such as quantifying fiber or ash to indicate pericarp and tip cap contents, and oil to indicate germ content. More direct and reliable methods are possible with tissue-specific markers. We used two transgenic maize lines, one containing the fluorescent protein green fluorescent protein (GFP) variant S65T expressed in endosperm, and the other containing GFP expressed in germ to determine the fate of each tissue in the dry-milling fractionation process. The two lines were dry-milled to produce three fractions (bran-, endosperm-, and germ-rich fractions) and GFP fluorescence was quantified in each fraction to estimate the tissue composition. Using a simplified laboratory dry-milling procedure and our GFP-containing grain, we determined that the endosperm-rich fraction contained 4% germ tissue, the germ-rich fraction contained 28% germ, 20% endosperm, and 52% nonendosperm and nonembryo tissues, and the bran-rich fraction contained 44% endosperm, 13% germ, and 43% nonendosperm and nonembryo tissues. GFP-containing grain can be used to optimize existing fractionation methods and to develop improved processing strategies.</p>
dc.description.comments <p>This article is published as Shepherd, C. T., N. Vignaux, J. M. Peterson, M. P. Scott, and L. A. Johnson. "Dry-Milling and Fractionation of Transgenic Maize Seed Tissues with Green Fluorescent Protein as a Tissue Marker." <em>Cereal Chemistry</em> 85, no. 2 (2008): 196-201, doi; <a href="https://doi.org/10.1094/CCHEM-85-2-0196">10.1094/CCHEM-85-2-0196</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/agron_pubs/142/
dc.identifier.articleid 1161
dc.identifier.contextkey 10425823
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath agron_pubs/142
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/4469
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/agron_pubs/142/2008_Scott_DryMilling.pdf|||Fri Jan 14 20:16:07 UTC 2022
dc.source.uri 10.1094/CCHEM-85-2-0196
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Breeding and Genetics
dc.title Dry-Milling and Fractionation of Transgenic Maize Seed Tissues with Green Fluorescent Protein as a Tissue Marker
dc.type article
dc.type.genre article
dspace.entity.type Publication
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