Mosquitoes are public health concerns as vectors of arthropod-borne viruses (arboviruses) and/or as nuisances to humans, so surveillance efforts are important to determine areas and times that may pose a risk to humans. The ISU Horticulture Research Station (HRS), Ames, Iowa, consistently yields mosquitoes that are positive for West Nile Virus (WNV). Objectives were to continue to assess human risk by monitoring mosquitoes, which are vectors that transmit this virus to people.