Developing real-time PCR assay for detection of multi-drug Salmonella strains in pigs
Date
2005-01-01
Authors
Robbins, Rebecca
Lowman, Heather
Gebreyes, Wondwossen
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Abstract
The main objective of this study was to develop a rapid, sensitive and accurate real-time detection assay for multi-drug resistant (MDR) Salmonella strains isolated from pigs. Initially, standardized procedures for use with real-time PCR using SYBR green were developed to evaluate selected primers, detection limitations using two predominant strains: S. typhimurium phage types DT104 and DT193.
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Sat Jan 01 00:00:00 UTC 2005