Developing real-time PCR assay for detection of multi-drug Salmonella strains in pigs

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2005-01-01
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Robbins, Rebecca
Lowman, Heather
Gebreyes, Wondwossen
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International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork
Iowa State University Conferences and Symposia

The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.

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The main objective of this study was to develop a rapid, sensitive and accurate real-time detection assay for multi-drug resistant (MDR) Salmonella strains isolated from pigs. Initially, standardized procedures for use with real-time PCR using SYBR green were developed to evaluate selected primers, detection limitations using two predominant strains: S. typhimurium phage types DT104 and DT193.

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Sat Jan 01 00:00:00 UTC 2005