Microbial production of bi-functional molecules by diversification of the fatty acid pathway

dc.contributor.author Garg, Shivani
dc.contributor.author Rizhsky, Ludmila
dc.contributor.author Jin, Huanan
dc.contributor.author Yu, Xiaochen
dc.contributor.author Jing, Fuyuan
dc.contributor.author Yandeau-Nelson, Marna
dc.contributor.author Nikolau, Basil
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.contributor.department NSF Engineering Research Center for Biorenewable Chemicals
dc.contributor.department Center for Metabolic Biology
dc.contributor.department Genetics, Development and Cell Biology
dc.date.accessioned 2022-03-23T15:08:59Z
dc.date.available 2022-03-23T15:08:59Z
dc.date.issued 2016-05
dc.description.abstract Fatty acids that are chemically functionalized at their ω-ends are rare in nature yet offer unique chemical and physical properties with wide ranging industrial applications as feedstocks for bio-based polymers, lubricants and surfactants. Two enzymatic determinants control this ω-group functionality, the availability of an appropriate acyl-CoA substrate for initiating fatty acid biosynthesis, and a fatty acid synthase (FAS) variant that can accommodate that substrate in the initial condensation reaction of the process. In Type II FAS, 3-ketoacyl-ACP synthase III (KASIII) catalyses this initial condensation reaction. We characterized KASIIIs from diverse bacterial sources, and identified variants with novel substrate specificities towards atypical acyl-CoA substrates, including 3-hydroxybutyryl-CoA. Using Alicyclobacillus acidocaldarius KASIII, we demonstrate the in vivo diversion of FAS to produce novel ω-1 hydroxy-branched fatty acids from glucose in two bioengineered microbial hosts. This study unveils the biocatalytic potential of KASIII for synthesizing diverse ω-functionalized fatty acids.
dc.description.comments This is a manuscript of an article published as Garg, Shivani, Ludmila Rizhsky, Huanan Jin, Xiaochen Yu, Fuyuan Jing, Marna D. Yandeau-Nelson, and Basil J. Nikolau. "Microbial production of bi-functional molecules by diversification of the fatty acid pathway." Metabolic engineering 35 (2016): 9-20. doi:10.1016/j.ymben.2016.01.003. Posted with permission. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/1wgePE9r
dc.language.iso en
dc.publisher Copyright © 2016 International Metabolic Engineering Society
dc.source.uri https://doi.org/10.1016/j.ymben.2016.01.003 *
dc.subject.keywords 3-Ketoacyl-ACP Synthase III
dc.subject.keywords fatty acid synthesis
dc.subject.keywords bio-based chemicals
dc.subject.keywords omega-functionalized fatty acids
dc.subject.keywords substrate diversity
dc.subject.keywords microbial engineering
dc.title Microbial production of bi-functional molecules by diversification of the fatty acid pathway
dc.type Article
dspace.entity.type Publication
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